传统活性污泥在抗生素去除过程中发挥重要作用，但是仍有部分抗生素难以被有效去除，而硝化污泥中的微生物在氨氮转化时通过共代谢模式能有效去除难降解的抗生素，因此值得人们深入研究。但是在该系统中，抗生素在不同微生物之间的分配和降解机制以及不同微生物之间的相互作用对抗生素去除的影响机制仍未清楚。因此，本研究针对硝化污泥系统中抗生素去除机理不清、微生物种间相互作用机制不明的问题，拟通过酶学分析方法，解析典型抗生素－甲氧苄氨嘧啶（TMP）在硝化污泥系统中不同微生物间的去除机理；利用14C同位素示踪技术确定系统中不同微生物间的相互作用机制以及对TMP降解的影响机理；结合分子生物学分析技术方法和活性污泥数学模型，优化反应器运行，强化抗生素去除，从而为污水中抗生素的高效去除提供理论基础和支持。

Although conventional activated sludge in wastewater treatment plants (WWTPs) play an important role in antibiotics removal, some antibiotics are recalcitrant to biodegrade in WWTPs. Since nitrifying activated sludge (NAS) can biodegrade these recalcitrant antibiotics efficiently through cometabolic mode during ammonium transformation process, it is worth to exploring deep in NAS systems. However, in this system, the roles of different microorganisms played in antibiotics biodegradation and the interaction effects between different microorganisms are unknown. Therefore, in this study, enzymatic analysis method was adopted to explore the mechanism of a typical antibiotic-TMP removal in NAS system by different microorganisms. 14C isotope tracer technology was used to clarify the interaction effect between different microorganisms and quantify the effect on TMP removal. Furthermore, molecular biology technology and activated sludge models (ASMs) were used to optimize the performance of biological reactor for TMP removal. Hopeful, this study can provide a theoretical knowledge and support for the antibiotics removal in wastewater treatment.