果实表面微生物组与环境通过物质、能量和信息交换，相互作用构成一个整体，参与了果实采后生理和病理过程。目前，采后病害生物防治的研究主要集中在果实、病原菌和生防菌三者的互作关系上，忽视了果实表面微生物组的作用。蓝莓果实采后极易遭受病原菌的侵染，造成巨大的经济损失。因此，本项目拟借助宏基因组学技术、扩增子测序技术结合传统的微生物分离培养鉴定技术系统解析蓝莓果实表面微生物组的特征，并探究果实贮藏过程中表面微生物群落对其采后主要病害的响应情况。在此基础上，定向筛选具有潜在生防功效的核心菌群，研究核心菌群对蓝莓果实表面微生物群落结构的重建能力。最终，揭示蓝莓果实表面微生物组调控其采后主要病害发生的机制。本项目的研究结果将为开发基于微生物组的果实采后病害生物防治技术提供新的理论基础和实践途径。

The fruit surface microbiome interacts with the environment through the exchange of substances, energy and information to form a whole. It participates in the postharvest physiological and pathological processes of fruit. At present, studies on biological control of postharvest diseases mainly focus on interactions among fruit, pathogen and biocontrol agents, but ignore the role of the fruit surface microbiome. Blueberry fruits are extremely susceptible to infection by various pathogens after harvest, resulting in huge economic losses. Therefore, this project intends to analyze the characteristics of the microbiome on the surface of blueberry fruit and explore the response of surface microbial communities to major postharvest diseases during fruit storage through metagenomics technology, amplicon sequencing technology combined with traditional microbial separation and culture identification technology. On this basis, the core microbial communities with potential biocontrol effects are screened. The ability of the core microbial communities to reconstruct the microbial community structure on the surface of blueberry fruit is investigated. Finally, the mechanism of blueberry fruit surface microbiome regulating the occurrence of its major postharvest diseases is revealed. The results of this project will provide a new theoretical basis and practical approach for the development of the biological control technology for postharvest diseases of fruit based on microbiome.