合理利用氮肥是减肥增效中亟待解决的问题。硝态氮（NO3–）是草莓最主要的氮源，而氮肥的大量施用不仅增加了种植成本，而且降低了氮肥利用率。因此，研究提高NO3–利用的基因是减肥增效的关键科学问题。研究表明AtNRT1.1/NPF6.3能转运和感知NO3–，钼酸盐转运蛋白MOT1可以吸收分配钼酸根（MoO42-），参与NO3–的同化利用途径。申请人前期研究发现缺Mo条件下草莓表现出氮缺乏症状，MoO42-调控NRT1.1的表达，NO3–则诱导了MOT1的表达，推测NRT1.1和MOT1可能共同调控NO3–的吸收利用。为此，我们利用分根系统和同位素标记(99MoO42-和15NO3–)研究MoO42-和NO3–的吸收利用的相互影响，克隆了草莓中的FaNRT1.1和FaMOT1基因，通过生理生化分析和转基因手段进一步研究两者对NO3-的吸收利用的调控，为提高草莓的氮素利用率提供新的分子生理依据。

It’s urgent to solve the problem how to use nitrogen fertilizer rationally. Nitrate (NO3–) is the main nitrogen source of strawberry. The excessive application of nitrogen fertilizer not only increases the cost of planting, but also reduces the utilization of nitrogen fertilizer and pollutes the environment. So it is important for studying the genes to reduce fertilizer use and improve quality. NRT1.1/NPF6.3 is demonstrated to act as a transceptor (transporter and receptor) for NO3– and it can transport and sense NO3–. Molybdate transporter MOT1 can be involved into the assimilation and distribution process of MoO42- and participates in the NO3–assimilation process. In our preliminary studies, strawberries showed symptoms of nitrogen deficiency in the Mo deficiency condition, and MoO42- can regulate the expression of NRT1.1 and MoO42- induces the expression of MOT1. It is speculated that NRT1.1 and MOT1 may jointly regulate the absorption and utilization of NO3–. Therefore, we will study the interaction between the absorption and utilization of MoO42- and NO3– using the split-root system and isotopic labeling (99MoO42- and 15NO3–). We have cloned FaNRT1.1 and FaMOT1and will confirm the regulation of the absorption and utilization of NO3– through physiological and biochemical analysis and transgenic research, which will provide new molecular and physiology basis for improving the nitrogen utilization of strawberries.