单细胞水平揭示RNA结合蛋白在造血干细胞分化中的功能与机制

RNA结合蛋白对于RNA的合成、剪接、定位和翻译等调控进程都具有关键作用，然而RNA结合蛋白在造血干细胞（HSC）分化中的系统认识仍不完善。因此，在前期整理的单细胞数据与分析平台基础之上，本项目拟综合利用单细胞转录组测序、机器学习算法与生物信息分析技术和体内外实验等方法，建立单细胞水平小鼠成体HSC分化和RNA结合蛋白分析平台，系统解析RNA结合蛋白在小鼠成体HSC分化中的作用，呈现RNA结合蛋白在小鼠成体HSC分化中的动态表达模式，挖掘在小鼠成体HSC分化中重要时空表达特异的RNA结合蛋白，并揭示重要RNA结合蛋白调控小鼠成体HSC分化的功能和机制，不仅有利于深化RNA结合蛋白对HSC分化调控机制的理解和认识，同时也对阐明HSC分化尚未揭示的调控机制具有重要作用和意义。

RNA binding proteins (RBPs) act as a key role in multiple biological regulation processes, including RNA synthesis, splicing, localization and translation. However, the systematic understanding of RBPs in hematopoietic stem cell (HSC) differentiation remains elusive. Hence, according to single-cell data and analysis platform, this project intends to use single-cell RNA sequencing, machine learning algorithms and bioinformatics analysis techniques, and in vitro and in vivo experiments to establish mouse adult HSC differentiation and RBPs analysis platform. Then, it will systematically decipher dynamic expression patterns of RBPs, and identify important RBPs during mouse adult HSC differentiation. Finally, the underlying regulation mechanism and function of these RBPs during mouse adult HSC differentiation will be revealed. It is not only helpful to deepen the understanding for the molecular mechanisms of RBPs in HSC differentiation, but also has important role and significance to clarify the unknown regulation mechanism of HSC differentiation.