胚胎发育早期造血干细胞数量非常稀少、存在时间相对短暂并且较难有效的和其他祖细胞分离，导致人们对自噬在胚胎造血干细胞发生过程中的作用知之甚少。为此，在前期筛选的胚胎造血干细胞发生过程中具有代表性的5类单细胞群体基础之上，本项目拟系统地构建基于单细胞水平胚胎造血干细胞和自噬相关数据资源与分析平台，进而分析自噬与胚胎造血干细胞发生过程的关联，系统地揭示自噬相关基因（蛋白编码基因和长非编码RNA）在胚胎造血干细胞发生过程中的动态变化规律，识别胚胎造血干细胞发生过程中关键自噬相关基因。从分子、细胞、动物等水平，揭示关键自噬相关基因调控胚胎造血干细胞发生的分子机制，不仅对阐明哺乳动物胚胎造血干细胞的体内发生和特化、形成和维持的分子机制有着重要意义，同时也为造血干细胞的体外再生等研究提供新的研究线索、思路和理论依据。

Because of embryonic hematopoietic stem cells (HSCs) rareness, transience and difficulty to achieve efficient isolation from other progenitor cells, the mechanism of autophagy during embryonic HSC formation remains elusive. Hence, according to 5 population cells during embryonic HSC formation, this project firstly intends to establish embryonic HSC and autophagy-related data resource and analysis platform at single-cell level, providing a critical insight into the mechanistic understanding of the physiological connections between autophagy and embryonic HSC formation. Then, it will systematically represent dynamic expression patterns of autophagy-related genes (protein-coding and long non-coding genes), and identify critical autophagy-related genes during embryonic HSC formation. Further, at molecular, cell and animal level, it will reveal the underlying regulation mechanism of these genes during embryonic HSC formation. It is not only of great significance to decipher the molecular mechanisms of mammalian embryonic HSC’s formation, specialization and maintenance in vivo, but also provide new orientation and theoretical basis for regeneration of HSCs in vitro.