昆虫体表非挥发性或低挥发性性信息素在两性近距离求偶过程中发挥重要作用。许多昆虫求偶时，表达于足上的化学感受相关蛋白参与了对近距离性信息素的感受。本申请以绿盲蝽为研究对象，通过HPLC、GC-MS、电生理及行为学检测等技术鉴定雌雄绿盲蝽成虫体表具有电生理和行为活性的化合物组分；利用扫描及透射电镜鉴定绿盲蝽足部化学感器类型，结合转录组测序及组织表达谱分析筛选足特异表达的化学感受相关基因；体外重组表达化学感受相关蛋白并利用结合实验及双电极电压钳筛选其配体化合物；RNAi结合电生理及求偶行为学解析化学感受相关蛋白识别低挥发及非挥发性性信息素的功能特征，阐明绿盲蝽足近距离感受性信息素的分子机制，揭示非挥发性或低挥发性性信息素对绿盲蝽短距离求偶行为的作用，为设计新型性信息素调控化合物及干扰昆虫交配策略提供理论依据。

The nonvolatile or low volatile sex pheromones on insect body surface play important roles in close-range courtship. The chemosensory proteins expressed in legs may be involved in perception of close-range sex pheromones in insect mating. Present study will be conducted to comprehend the molecular basis of Apolygus lucorum legs in perception of close-range sex pheromones. Specific objectives are: using HPLC, GC-MS, combined with electrophysiological and behavioral techniques to identify the compounds released from insect body surface, which can trigger electrophysiological and behavioral responses of A.lucorum in mating behavior; identifying the sensilla types on insect legs by using scanning and transmission electron microscope; screening the specific chemosensory genes expressed in legs by using RNA-seq and RT-qPCR methods; investigating the interactions between recombinant chemosensory proteins and the candidate ligands by using ligand-binding assays and two-electrode-voltage-clamp recordings; verifying the roles of A.lucorum legs in perception of close-range sex pheromones by RNAi as well as electrophysiological and behavioral tests in vivo. Our data will provide theoretical basis for developing new compounds to regulate the mating behavior of mirid bugs.