微管蛋白是生物细胞凋亡多肽作用的直接靶点。高氧、低氧和高二氧化碳等均可调控果蔬采后衰老，特别是相关蛋白质组表达，其中高氧短时处理更加简易实用。本项目以经典非呼吸跃变型果实草莓为试材，研究80%–100%高氧短激1-24h对草莓微管蛋白差异表达水平的调控作用，发现高氧调节微管蛋白表达上调或下调的作用变量；研究高氧对微管蛋白聚合-解聚动力学特性的介导效用，并预测微管蛋白可能的磷酸化修饰及其二级结构；结合草莓细胞衰老的超微结构及品质参数变化，进一步量化分析各参数和变量之间的相关性，构建“高氧介导→微管蛋白表达调控→延迟或回避果实细胞衰亡”动态模拟理论并进行验证。本研究采用“三结合”技术策略，旨在从分子生物学水平揭示细胞衰老过程中微管蛋白的表达机理，对开发低碳、绿色的果蔬保鲜措施，具有非常重要的意义；基于分子水平为Proteomics to Preservative研究高氧介导理论提供依据。

Tubulin is the direct target of peptides in biological cell apoptosis. The high oxygen, low oxygen or high carbon dioxide treatment could regulate postharvest senescence of fruit and vegetable, especially the expression level of proteomics. However, the short-term treatment of high oxygen is quite simple and effective. In the present study, the classic non-climacteric fruit, strawberry, was treated by 80%-100% O2 for 1-24h, and the up-regulated or down-regulated expression level of tubulin was monitored, in order to get the variable role of oxygen concentration and time; the polymerization-depolymerization processes and dynamics of tubulin were studied, and the possible phosphorylation modification and its secondary structure were investigated in strawberry. The correlations between the above and microstructure as well as quality parameters of strawberry were also calculated in order to build and verify a dynamic simulation of ‘high oxygen – tubulin expression – avoiding cell senescence’. Three ‘combinations’ strategy was used in this study to elucidate the molecular mechanism of tubulin expression in cell senescence regulated by high oxygen for a short time. This study could help develop an environmental and green preservative technology as well as research a ‘proteomics to preservative’ on a molecular basis, which is of great insignificance and provide the evidence for high oxygen theory.