RAP2.12在外源氧诱导草莓脂肪族化合物代谢过程中的功能解析

风味化合物的代谢及其调控机制是食品科学领域值得关注的科学问题。但目前基于亚细胞水平的蛋白质和转录因子介导风味代谢的研究相对滞后。前期研究以八倍体草莓为研究对象，筛选到外源氧响应蛋白FaRAP2.12定位于细胞核。本项目以外源氧诱导条件下FaRAP2.12在草莓挥发性脂肪族化合物（VAC）代谢过程中的分子调控作用为核心问题，开展FaRAP2.12生物学特性和细胞核蛋白质组学研究，利用酵母杂交、双分子荧光互补、免疫共沉淀、共定位试验、基因沉默技术结合生物信息学功能分析，旨在揭示外源氧信号诱导FaRAP2.12参与VAC代谢的作用方式和调控机制。本研究有助于构建非生物胁迫因子下草莓VAC代谢的稳态机制，研究结果将丰富亚细胞转录后水平上草莓品质组分代谢调控理论，为精准调控果品采后品质提供理论依据和技术支撑。

The metabolic and regulation mechanism of fruit flavor compounds is an important scientific issue in food science. Nevertheless, there is relatively little research on flavor metabolism mediated by proteins or transcription factors at subcellular level so far. On the basis of our previous research, we screened the protein FaRAP2.12, in response to exogenous oxygen, was located in the nucleus of strawberry (Fragaria × ananassa Duch.). In the present project, we will focus on the molecular role of RAP2.12 involved in the metabolism of volatile aliphatic compounds (VAC) in response to exogenous oxygen induction, as well as investigate the FaRAP2.12 biological characteristics and nucleus proteomics. In addition, the yeast two-hybrid (Y2H), bimolecular fluorescence complementarity, co-immunoprecipitation (Co-IP), co-localization test, gene silencing technology and bioinformatic function analysis will be carried out in order to excavate the role of FaRAP2.12 involved in the VAC metabolism and regulation in response to exogenous oxygen signal induction. The present study will help to construct the homeostasis mechanism of VAC metabolism in strawberry under abiotic stress stimuli. Results from the present study will not only enrich the molecular theory of strawberry quality components at the subcellular post-transcriptional level, but also provide the theoretical basis and technical support for the accurate regulation of fruit postharvest quality.