卤代苯醌（HBQs）是饮用水中新现的一类未受控消毒副产物，结构定量毒性关系模型分析可能是膀胱癌致癌物，然而目前不仅对HBQs的形成还是致癌性均缺乏直接实验数据。故本研究基于质谱多反应检测（MRM）技术，对南宁市饮用水中HBQs的状况进行调查，研究水源水质、制水工艺、消毒剂种类和管网输送对HBQs形成的影响，并构建HBQs生成和转归预测模型。继而通过一套反映基因突变、DNA损伤和染色体断裂等多遗传终点的毒性测试组合方法以及体外细胞恶性转化试验对HBQs的遗传毒性和潜在致癌性进行评估，并从Nrf2- Keap1- ARE通路探讨其作用机制。研究结果将为水厂自来水加工工艺优化升级、HBQs的过程控制和制定饮用水水质卫生标准提供科学依据。

Halobenzoquinones (HBQs) are a new class of emerging unregulated disinfection by-product in drinking water. Quantitative structure toxicity relationship (QSTR) analysis predicted HBQs as plausible bladder carcinogens. However, there is little or no experimental data on the formation and carcinogenicity of these compounds. Thus, this study aims to carry out a comprehensive study on the formation of HBQs in drinking water of water plants of Nanning based on mass spectrometry multiple reaction monitoring (MRM) method. The relationship between water quality/treatment/distribution and HBQs formation will be investigated. The predictive models for HBQs formation and transformation will be made based on these surveillance data. The genotoxicity of HBQs will be evaluated by a battery of genetic bioassays covering gene mutation, DNA damage, and chromosome aberration. The potential carcinogenicity of HBQs will be determined by cell transformation assay in vitro. Further, the role of Nrf2- Keap1- ARE pathway in the toxical mechanism will be studied. The data obtained will be valuable to water suppliers for water treatment optimization and HBQs control. It will be also helpful to provide evidence for the establishment of standard for drinking water quality.