牙周炎是一类以牙周支持组织破坏为特征的慢性感染性疾病，是免疫系统功能异常引发骨质破坏的疾患之一。将免疫抑制剂和牙周组织工程技术联合应用治疗牙周炎的研究目前尚未见报道。本项目组前期已成功构建多种纳米粒，并利用静电纺丝技术构建复合纳米支架，采用牙周组织工程技术有效地促进了骨组织再生。在此基础上，本项目拟应用同轴静电纺丝技术构建具有芯壳结构的纳米纤维，以纳米粒包裹免疫抑制剂使其分散在壳层结构，同时细胞生长因子分散在芯层结构。通过修饰免疫抑制剂和生长因子所在纤维的结构等手段来调节释放顺序和浓度，使其先释放免疫抑制剂后释放生长因子从而达到靶向控释的作用，将消炎和促再生有效地结合起来治疗牙周炎，同时从JNK/ERK/p38 MAPK 通路分析对炎症相关因子的表达情况，揭示其分子机制，并进一步探寻有针对性的治疗方法。

Periodontitis as a chronic infectious diseases, was characterized by periodontal tissue destruction, which was caused by microenvironmental immune hyperthyroidism. No related report was found which combining immunosuppressants and periodontal tissue engineering. We already successfully constructed nanoscaffolds by electrospinning technology with variety kind of nanoparticles, which effectively promote bone tissue regeneration. We aim to build a dimensional constructed nanoscaffold, with nanoparticles wrapping dispersed immunosuppressants as shell while the cell growth factor as core. By means of modifying the nanofiber structure, the releasing order and concentration of the immunosuppressants and growth factors was sequentially and targeted controlled, to achieve the anti-inflammatory and pro-regenerative effectively combined treatment of periodontal disease. Meanwhile, we will further study the expression of the inflammatory cytokines , from the perspective of JNK/ERK/p38 MAPK pathway, and explore more efficacious treatment.