拟南芥在高盐胁迫下会产生活性氧，而过多的活性氧会对植株造成伤害。过氧化物酶体含有可以消除活性氧的过氧化物酶、过氧化氢酶等。植物细胞中过氧化物酶体的发育和运动，活性氧的平衡与细胞骨架的动态是密切相关的，但是三者在植物适应盐胁迫的过程中是如何协同起作用的仍有待深入解析。申请人前期的研究证明了拟南芥QWRF1蛋白可以结合微管并调控微管的排列组织，其突变体活性氧含量增加且对高盐胁迫敏感。本项目拟分析QWRF1是如何通过调控微管骨架的动态来影响过氧化物酶体发育及其抗氧化酶的活性进而调节了高盐胁迫下活性氧的平衡，使得拟南芥植株增强盐胁迫下的适应性。本项目提出了从微管系统和过氧化物酶体发育的协同互作研究植物耐受盐胁迫机制的新思路，研究结果将提出植物适应盐胁迫的新的细胞生物学机制，对深入了解植物适应盐胁迫的调控机理有重要的科学理论意义。

In plants, salt stress causes an increase in the intracellular level of reactive oxygen species (ROS), leading to tissue injury. Plant peroxisomes maintain key life processes including homeostasis of ROS. The movement and development of peroxisomes in plant cells are dependent on microtubules cytoskeleton and closely related to the production and scavenging of ROS. In addition, recent evidences indicate that microtubules play a role in peroxisome proteins import. However, the relationship of cortical microtubules arrangement, peroxisomes and ROS homeostasis during plant adaptation to salt stress remains largely unknown. Through genetic screening of Arabidopsis thaliana mutants that are sensitive to salt stress, we identified QWRF1 as a candidate negative regulator of reactive oxygen species (ROS) in response to salt stress. QWRF1 is a member of a largely uncharacterized protein family QWRF unique to the plant kingdom. Our studies have shown that the QWRF family of Arabidopsis thaliana co-localize with cortical microtubules. Research objective of this project is to identify QWRF1 as a protein dually targeted to cortical microtubules and peroxisomes. QWRF1 targeting peroxisomes by PTS1 under salt stressful conditions is depended on microtubules, and is helpful to eliminate ROS and improving plant adaption to salt stress.