植物成花调控是当前国内外研究的热点课题，FLOWERING LOCUS T（FT）基因位于成花调控的关键位置，是植物成花的关键促进因子。芒果是亚热带地区重要的经济果树之一。前期研究中，我们从芒果成花不同发育时期的转录组数据中挖掘获得3个FT同源基因，这3个FT基因的表达模式与芒果成花密切相关。由于芒果的成花特性与模式植物拟南芥存在明显差异，FT基因是如何调控芒果成花的呢？其分子机制尚不清楚。本项目拟在前期研究的基础上，通过基因表达模式和转基因功能分析，探明3个FT基因在芒果成花中的功能，明确芒果成花的关键FT基因，通过关键FT基因启动子截短体活性分析，鉴定启动子主要顺式作用元件的功能，通过酵母杂交等技术筛选和鉴定FT基因的互作蛋白，并分析这些互作蛋白与芒果成花的关系，以期阐明芒果FT基因在成花过程中的功能和调控机制。项目的实施将为芒果花期调控提供理论依据，丰富植物成花调控理论。

Plants flowering regulation is currently a hotspot of research topic. The FLOWERING LOCUS T（FT）gene is located in the key position of flower regulation, which is the key promoter of flowering in plants. Mango（Mangifera indica L.）is one of the most important economic fruit trees in the subtropical region. In our preliminary study, three full-length FT homologous genes were obtained from the transcriptome data of mango samples which collected at different flowering development stages. The expression patterns of these genes showed closely related to mango flowering. However, the flowering characteristics of mango were significantly different from that of the model plant Arabidopsis. How did the role and function of this three FT genes in the process of mango flowering? The molecular mechanism is not clear and necessary to be further studying. We will focus on the expression pattern and function analysis the three FT genes in mango based on our previous studies. Clarify the function of the three FT genes during mango flowering and obtained the key flowering gene. Identified the main cis-acting element function of the key FT gene by truncated promoter activity analysis. Screening and identification the interaction protein by using yeast hybrid systems. Furthermore, we will analysis the relationship between these interaction proteins and mango flowering. The goal of this project was to investigate the function and regulation mechanism of FT gene in the reproductive growth process of mango. The results of this project will provide theory basis for mango flowering regulation and enrich plant flowering regulation theory.