发展高效的肝癌早期诊断方法是提高肝癌患者生存率的关键。基于荧光探针的荧光成像技术因具有灵敏度高、选择性好等优势，在肝癌的早期诊断领域展现出诱人的应用前景。但是现有荧光探针存在信号发射波长较短、生物背景干扰严重、斯托克斯位移较短、激发光干扰等不足，限制了其在活体检测中的应用。针对以上不足，本项目拟将化学发光与近红外成像技术相结合，构建基于能量共振转移的化学发光近红外检测平台用于肝癌的早期诊断。拟采用化学发光基团作为能量供体，近红外发光基团作为能量受体，实现在无激发光下的长波长发射，解决活体成像过程中生物背景干扰和激发光干扰等问题；针对肝癌组织特异性高表达生物酶的特点，拟引入特异性识别基团，发展灵敏度高、特异性好、分辨率高和组织穿透深度大的近红外化学发光探针，为肝癌的早期诊断提供新方法。

Developing effectively early diagnosis method is great important to improve the prognosis of hepatic carcinoma and patient survival. Fluorescent imaging technique based on fluorescent probe provides an ideal method for hepatic carcinoma highly selective and sensitive detection. However, these fluorescent probes for hepatic carcinoma exist several problems such as high biological interference caused by short luminescence wavelength, and large excitation light interference caused by short stocks shift. Therefore, this project attempt to develop a resonance energy transfer based chemiluminescence near-infrared detection system by combining the chemiluminescence dye and near-infrared dye. Exciting the near-infrared dye, which is the energy acceptor, by chemiluminescence energy from chemiluminescence dye instead of exciting laser, could avoid excitation light interference and biological background during imaging in vivo system. Recognition groups, which developed according these biological enzymes over expressed in hepatic carcinoma environment, are introduced into the near-infrared chemiluminescence system for highly sensitive and real-time hepatic carcinoma detection. The completion of this project will provide reference and theoretical basis for hepatic carcinoma early diagnosis.