深入了解油菜菌核病的抗病机制对有效促进油菜抗菌核病育种有重要意义。现有油菜中缺乏有效的菌核病抗源，限制了对其抗病机制的研究与应用。本课题组前期研究发现一份野生甘蓝（Brassica incana）对核盘菌有突出抗性，并对其进行了抗病QTL定位。我们以感病亲本为轮回亲本，结合分子标记辅助回交，构建了抗、感分离的BC3F2群体。为研究该高抗菌核病野生甘蓝的抗病机理，本项目申请拟在BC3F2中构建抗、感病混合池，运用蛋白质组技术鉴定接种前后抗、感混合池的差异蛋白，并将其编码基因锚定到甘蓝参考基因组上，结合前期QTL定位结果获取抗性相关基因，并在BC3F2群体中进行DNA和转录水平上的验证，筛选候选基因；同时我们还将通过生物信息学分析解析该野生甘蓝的抗病机理。该项目的实施将有助于深入研究野生甘蓝抗菌核病机理，丰富菌核病抗性理论，为利用高抗病野生甘蓝改良油菜菌核病抗性奠定理论基础。

Uncovering the mechanism involved in the resistance against Sclerotinia sclerotiorum rot (infected by Sclerotinia sclerotiorum) in oilseed rape is very important to stimulate breeding focusing on resistance improvement. However, the lack of resistant resource in cultivated species has dramatically impeded the related research and the corresponding application. Fortunately, our group have discovered Brassica incana has more excellent resistance compared with ZhongYou 821, commonly recognized as tolerance.We mapped QTL for Sclerotinia resistance in it. We created BC3F2 population using the suceptible one as recurrent parent with the assistance of MAS. To more deeply understand the mechanism of resistance, we will construct resistant pools and susceptible ones using the most resistant individuals and the most susceptible ones in BC3F2 population; investigate the proteome change resulting from S. sclerotiorum challenge; locate the corresponding coding genes on the reference genome of B. oleracea; determine the resistance-related genes based on the QTL analysis results in our group; and screen out the candidated resistance genes based on the verfication of genome and transcriptome level. Finally, we will duscuss the resistance mechanism of B. incana. The implement of the project will be beneficial to deeply understand the resistance mechanism in B. incana and enrich the resistace theory, laying the foundation for the utilization of the resistance genes in B.oleracea to breed B. napus with higher level resistance against S. sclerotiorum.