Transcriptomic comparison between Brassica oleracea and rice (Oryza sativa) reveals diverse modulations on cell death in response to Sclerotinia sclerotiorum.
Transcriptomic comparison between Brassica oleracea and rice (Oryza sativa) reveals diverse modulations on cell death in response to Sclerotinia sclerotiorum.
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甘蓝和水稻 (Oryza sativa) 之间的转录组比较揭示了核盘菌对细胞死亡的不同调节作用
DOI：
10.1038/srep33706
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发表时间：
2016-09-20
影响因子：
4.6
通讯作者：
Qian W
中科院分区：
文献类型：
--
作者：
Mei J;Ding Y;Li Y;Tong C;Du H;Yu Y;Wan H;Xiong Q;Yu J;Liu S;Li J;Qian W
由核盘菌（Sclerotinia sclerotiorum）引起的菌核病（Sclerotinia stem rot）是芸苔属作物的毁灭性病害，但在水稻中不存在。以一个水稻株系、一个部分抗性（R）和一个部分感病（S）的甘蓝（Brassica oleracea）群体的叶片为材料，在接种S. sclerotiorum培养6和12 h。在B之间显示不同的转录组谱。oleracea和水稻对S.菌核。GO和KEGG的富集分析表明R B中的抗氧化活性增强。组织化学染色显示，水稻和R B中活性氧（ROS）积累和细胞死亡明显减轻。与S B相比，甘蓝。在S B中检测到显著增强的Ca 2+信号传导，其是ROS和细胞死亡的正调节剂。而在R B中则受到显著抑制。甘蓝组。两种B组间差异有显著性。甘蓝组WRKY转录因子，特别是那些调节细胞死亡。这些结果表明，在宿主细胞死亡响应S。菌核本研究为深入了解番茄对S.菌核
Sclerotinia stem rot caused by Sclerotinia sclerotiorum is a devastating disease of Brassica crops, but not in rice. The leaves of a rice line, a partial resistant (R) and a susceptible (S) Brassica oleracea pool that bulked from a resistance-segregating F2 population were employed for transcriptome sequencing before and after inoculation by S. sclerotiorum for 6 and 12 h. Distinct transcriptome profiles were revealed between B. oleracea and rice in response to S. sclerotiorum. Enrichment analyses of GO and KEGG indicated an enhancement of antioxidant activity in the R B. oleracea and rice, and histochemical staining exhibited obvious lighter reactive oxygen species (ROS) accumulation and cell death in rice and the R B. oleracea as compared to that in the S B. oleracea. Significant enhancement of Ca2+ signalling, a positive regulator of ROS and cell death, were detected in S B. oleracea after inoculation, while it was significantly repressed in the R B. oleracea group. Obvious difference was detected between two B. oleracea groups for WRKY transcription factors, particularly for those regulating cell death. These findings suggest diverse modulations on cell death in host in response to S. sclerotiorum. Our study provides useful insight into the resistant mechanism to S. sclerotiorum.
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