转移是影响鼻咽癌（NPC）预后的重要因素。近来研究提示，肿瘤血管生成与NPC转移密切相关，但其机制研究尚不充分。本项目进一步研究了NPC血管生成与转移间的关系，并发现有转移的NPC患者来源的外泌体中HAX-1明显高于未转移者。结合课题组前期发现NPC来源的外泌体中HAX-1能促进血管生成，本项目提出：外泌体中富含的HAX-1可促进NPC血管生成，影响患者转移。本课题拟通过免疫组化等方法进一步研究肿瘤血管生成与NPC转移的关系；采用CCK8、Transwell、流式细胞术及小管形成实验分析不同来源的外泌体对血管生成的影响，明确外泌体中的HAX-1在其中所起的作用；并在转基因斑马鱼模型中，应用基因编辑和高分辨率活体成像等技术深入研究HAX-1促血管生成的细胞和分子机制；最后通过斑马鱼模型及临床大样本验证HAX-1促血管生成与转移的关系。本研究为NPC预后判断及精准治疗提供新的科学依据。

Metastasis is an important factor that affect the prognosis of NPC patients. Previous studies have shown that tumor angiogenesis exerts an important role in NPC metastasis, however，the mechanisms remain unknown. In the present study, we found that tumor angiogenesis promote the metastasis of NPC; HAX-1 was overexpressed in exosomes derived from NPC patients with metastasis（metastasis-exosomes） compared with non-metastasis （nonmetastasis-exosomes）. What's more, lately we have been reported that NPC derived exosomes are enriched in HAX-1 and promote NPC metastasis. In view of this, we speculate that exosomal HAX-1 could promote NPC angiogenesis, thus promote the metastasis of NPC. In this study, firstly we aim to explore the impact of tumor angiogenesis on NPC metastasis by Immunohistochemistry. Secondly, we will determine the impact of metastasis-exosomes and exosomal HAX-1 on NPC angiogenesis by CCK8, transwell, flow cytometry and tube formation experiment. Furthermore, we will verify the molecular mechanism of the way HAX-1 promotes angiogenesis using the Talen and morpholin method in zebrafish model. Finally, the role of HAX-1 promoting NPC metastasis will be validated through the zebrafish model and clinical large sample. Our study can provide new scientific basis for NPC prognosis judgments and provide accurate treatments for NPC therapy.