一种评价LNG浓度梯度的仿子宫透明体模的建立

Our previous study showed that there existed a "therapeutic threshold" for treatment adenomyosis by levonorgestrel-releasing intrauterine system (LNG-IUS). These phenomena indicated that the active ingredient in LNG-IUS would be presented as a kind of concentration gradient in the uterine wall. When the concentration of LNG drops to a certain value, the treatment effects of LNG to adenomyosis were lost.. LNG is a kind of powerful progesterone appears as white powder, its basic chemical structure is pregnane, side’s chain groups are: A ring C-3- keto, D ring C-17- hydroxyl group, D ring C-17- acetylene groups and so on. These structures could set off some chemical reaction with certain chemicals such as acid (sulfuric acid, phosphoric acid), carbonyl reagent (hydroxylamine, phenylhydrazine, isoniazid, semicarbazide etc) and silver nitrate, and present different colors. The special color reaction of LNG with different chemicals could be used to investigation and measurement the content of LNG indirectly..The purpose of this project is to use of this colorful reaction features of LNG with special chemical agents to formation a certain colored mark LNG (M-LNG), then put M-LNG into the cavity of the transparent imitation uterus model (TIUM), which mainly made with gelatin or polyacrylamide.The dispersion regularity of M-LNG in TIUM wall would be observed and measured by colorimeter in different follow up time point. There for, provide theoretical basis for the drug use of intrauterine treatment in clinic, meanwhile, for the further purpose of providing an experiment platform to research the intrauterine system systematically and deeply in the coming days.

前期研究结果表明，左炔诺酮宫内缓释系统（LNG-IUS）治疗子宫腺肌病存在“病灶阈值”现象，这使我们推测，其有效成份左炔诺酮（LNG）在子宫肌壁可能存在浓度梯度，当浓度递减至一定程度时，则会失去治疗效果。.LNG是强效孕激素，白色粉末状，基本化学结构为孕甾烷，侧链基团有：A环C-3-酮基、D环C-17-醇羟基；D环C-17-乙炔基等结构，这些结构能与酸（硫酸、磷酸）、羰基试剂（羟胺、苯肼、异烟肼、氨基脲）、硝酸银生等发生显色反应。利用LNG相关基团与不同化学试剂间的特殊显色反应，就能对LNG及其含量进行简接的观察、测量。.本项目即是利用LNG的这种特性，以特定化学试剂“显色标记”LNG，置于以明胶或聚丙烯酰胺为主体的仿子宫透明体模（TIUM）腔中，研究、观察显色后的LNG在TIUM壁中的弥散规律，为宫内药物治疗提供理论依据；同时，为今后系统、深入研究“宫内物药缓释系统”提供实验平台。

前期研究结果表明，左炔诺酮宫内缓释系统（LNG-IUS）治疗子宫腺肌病存在“病灶阈值”现象，这使我们推测，其有效成份左炔诺酮（LNG）在子宫肌壁可能存在浓度梯度，当浓度递减至一定程度时，则会失去治疗效果。LNG是强效孕激素，白色粉末状，基本化学结构为孕甾烷，侧链基团有：A环C-3-酮基、D环C-17-醇羟基；D环C-17-乙炔基等结构，这些结构能与酸（硫酸、磷酸）、羰基试剂（羟胺、苯肼、异烟肼、氨基脲）、硝酸银生等发生显色反应。利用LNG相关基团与不同化学试剂间的特殊显色反应，就能对LNG及其含量进行简接的观察、测量。本项目即是利用LNG的这种特性，以特定化学试剂“显色标记”LNG，置于以明胶或聚丙烯酰胺为主体的仿子宫透明体膜（TIUM）腔中，研究、观察显色后的LNG在TIUM壁中的弥散规律，为宫内药物治疗提供理论依据；同时，为今后系统、深入研究“宫内物药缓释系统”提供实验平台。

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