与动物细胞相比，植物体细胞具有高度全能性。已分化的植物体细具有再生成为其他类型组织器官和完整植物体的能力，但在正常生长发育中不会被激活。植物体细胞再生是一个复杂而有序的细胞重编程过程，涉及大量基因在不同时空水平的特异表达。然而，对影响体细胞再生的关键调控因子的鉴定及作用机制研究相对匮乏。此外，由于很多作物的遗传转化体系依赖体细胞再生，增强体细胞再生能力可提高作物遗传转化效率。因此，解析植物体细胞再生的分子机制既是重要的生物学问题，又具有很高的应用价值。本研究拟借助拟南芥原生质体再生系统，结合转录组学分析和基因编辑技术，通过反向遗传学筛选鉴定影响体细胞再生的关键基因，揭示植物细胞全能性的生物学基础，为作物遗传转化体系的优化改良提供新的思路。

Distinct from animals, plants can obtain a high totipotency in somatic cells. In plants, fully differentiated somatic cells maintain the competence of regenerating into other tissue or organ types, or even an entire plant. It has long been known that the regeneration process undergoes complex cell reprogramming which is tightly regulated, including the spatiotemporal expression of numerous regeneration-related genes. However, the identification and characterization of key regulators of somatic cell regeneration are still lacking. Moreover, since the transformation system of many crop species rely on somatic cell regeneration, increase of regeneration capacity may facilitate the transformation efficiency. Hence, dissecting molecular mechanisms underlying regeneration process is not only fundamental to biology, but has a wide range of applications. In our project, we aim to identify important regulatory genes during regeneration process of Arabidopsis protoplasts via a reverse genetic screen, combining both transcriptome analysis and genome editing. The results obtained from this project will provide a mechanistic framework to understand the biological basis of totipotency in plant somatic cells, and may offer new solutions in improving the transformation efficiency in crops.