硼是植物生长发育必需的微量元素之一。虽然被认定为必需元素到今已过去近一个世纪，我们仍不清楚缺硼导致生殖器官败育，即在生产实践中“花而不实”现象背后的分子机理。本项目基于前期鉴定到的一个在油菜花蕾中特异表达的硼转运蛋白BnaC06.NIP6;1，结合申请人上一站博后期间建立的油菜生殖器官缺硼培养体系，研究该基因在油菜生殖器官发育中的生物学功能和分子调控机制。主要内容为：1）通过蛙卵系统鉴定该基因对硼的转运能力；2）构建组织/亚细胞定位、过表达和CRISPR-Cas9载体转化油菜，验证基因功能，分析该基因对生殖器官不同组织间硼分配的影响；3）通过酵母单杂和双荧光素酶体系筛选调控目的基因表达的上游转录因子。研究结果有助于揭示缺硼条件下BnaC06.NIP6;1如何调控生殖器官不同组织间的硼分配以保证良好的生殖发育，还为作物硼营养的遗传改良提供硼高效基因和分子标记，有重要的理论和实践意义。

Boron is one of the essential microelements for plant growth and development. Although it has been identified as an essential element for about a century, we still do not know the molecular mechanism behind boron deficiency induced reproductive organ abortion in plants, which is also known as " flowering but without fruiting " in agricultural practice. After his first postdoc training, the applicant has gained skills of how to induce boron deficiency in reproductive organs of Brassica napus, and he is willing to initiate the project with a newly identified flower bud-specific boron transporter BnaC06.NIP6; 1 to characterize its function and regulation mechanism. Major research components are: 1) Demonstrating the functionality of BnaC06.NIP6;1 for boron transportation in oocyte; 2) Creating constructs for subcellular localization, overexpressing and CRISPR-Cas9 knockout lines to understand the function of BnaC06.NIP6;1, especially the role of BnaC06.NIP6;1 in distributing boron in reproductive organ under boron deficiency; 3) Based on yeast one-hybrid system and dual luciferase reporter assay, the project also aims to identify possible transcription factors that could regulate the expression of BnaC06.NIP6;1. The result will reveal how BnaC06.NIP6;1 regulates the distribution of boron between different reproductive tissues to ensure a proper reproductive development under boron deficient condition. This improvement will also demonstrate a new boron-efficient gene and provide a reliable molecular marker for boron research, which has both theoretical and practical significance.