温阳健脾法通过CREB/lnc-TMEM44/TLR4信号轴调控复发性流产的母胎免疫耐受能力的机制研究

复发性流产（RSA）是育龄期妇女妊娠常见的并发症之一，发病率呈逐年上升趋势，该病已从医学问题上升到影响社会稳定的严重问题。前期研究发现，温阳健脾法对RSA具有较好的临床疗效，前期研究发现保胎饮能调控Th17/Treg细胞平衡介导正常妊娠免疫耐受。但其本课题拟以CREB/lnc-TMEM44/TLR4信号轴为切入点，通过构建CBA/J×DBA/2小鼠RSA模型和CREB/lnc-TMEM44/TLR4信号轴基因细胞体外诱导分化模型，检测TCREB/lnc-TMEM44/TLR4信号轴细胞因子水平、基因表达、信号传导通路蛋白表达，研究温阳健脾法通过影响CREB/lnc-TMEM44/TLR4信号轴治疗RSA母-胎免疫耐受作用机制，以期为中药复方保胎饮治疗RSA的临床应用提供科学依据，为后续的新药研发奠定科学基础。

Recurrent Spontaneous Abortion(RSA) is a complicated disease in women of reprouductive age, and its morbidity rate is on rising year by ear.It has become a urgent problem, which is harmful to social stability. According to previous researches, significant efficacy of warming yang and strengthening spleen approch on RSA in clinical study are observed, however, detailed mechanisms of action are not investigated. Recent studies have new opinions that the balance of CREB/lnc-TMEM44/TLR4 signal axis cells is closely related to normal gestation immune tolerance, therefore, we put forward the hypothesis that Bao Tai Yin possess a satisfactory effect in treating RSA may mediate by regulation of CREB/lnc-TMEM44/TLR4 signal axis blance. In this research, in vivo CBA/J×DBA/2 mice RSA model and in vitro CREB/lnc-TMEM44/TLR4 signal axis differentiation model are established. CREB/lnc-TMEM44/TLR4 signal axis and their specific transcription factor and protein expressions are investigated. The theory that warming yang and strengthening spleen approch affects CREB/lnc-TMEM44/TLR4 signal axis to prevent RSA caused by maternal –fetal immune tolerance will be tested, which will provide scientific evidences for herbal compound Bao Tai Yin. Moreover, informations about new drug research and development in treating RSA will be provided.