基于Th17/Treg细胞平衡研究温阳健脾方治疗复发性流产母-胎免疫耐受作用机制

复发性流产（RSA）是育龄期妇女妊娠常见的并发症之一，发病率呈逐年上升趋势，该病已从医学问题上升到影响社会稳定的严重问题。前期研究发现，温阳健脾方对RSA具有较好的临床疗效，但缺乏确切的作用机制，结合现代医学有关Th17/Treg细胞平衡与正常妊娠免疫耐受密切相关的新认识，提出了“温阳健脾方可能通过调控Th17/Treg细胞平衡介导正常妊娠免疫耐受，从而实现良好治疗作用”的假说。本课题拟以Th17/Treg细胞平衡为切入点，通过构建CBA/J×DBA/2小鼠RSA模型和Th17/Treg细胞体外诱导分化模型，检测Th17/Treg细胞因子水平、特异转录因子表达、信号传导通路蛋白表达，研究温阳健脾方通过影响Th17/Treg细胞平衡治疗RSA母-胎免疫耐受作用机制，以期为中药复方温阳健脾方治疗RSA的临床应用提供科学依据，为后续的新药研发奠定科学基础。

Recurrent Spontaneous Abortion(RSA) is a complicated disease in women of reprouductive age, and its morbidity rate is on rising year by year.It has become a urgent problem, which is harmful to social stability. According to previous researches, significant efficacy of Wenyangjianpi prescription on RSA in clinical study are observed, however, detailed mechanisms of action are not investigated. Recent studies have new opinions that the balance of Th17/Treg cells is closely related to normal gestation immune tolerance, therefore, we put forward the hypothesis that Wenyangjianpi prescription possess a satisfactory effect in treating RSA may mediate by regulation of Th17/Treg cells blance. In this research, in vivo CBA/J×DBA/2 mice RSA model and in vitro Th17/Treg cells differentiation model are established. The balance of Th17/Treg cells and their specific transcription factor and protein expressions are investigated. The theory that Wenyangjianpi prescription affects Th17/Treg cells balance to prevent RSA caused by maternal -fetal immune tolerance will be tested, which will provide scientific evidences for herbal compound Wenyangjianpi prescription. Moreover, informations about new drug research and development in treating RSA will be provided.