研究表明SUMO-1基因和恶性肿瘤发生发展关系密切。我们前期研究发现在肝癌标本及肝癌细胞中，SUMO-1基因均显著高表达。通过RNAi方法，抑制HCCLM3中 SUMO-1基因表达，划痕实验、Transwell侵袭实验显示细胞迁移及侵袭能力下降，表明SUMO-1基因和肝癌的侵袭与转移有关。本项目拟通过RT-PCR、western blot及免疫组化进一步研究SUMO-1基因和肝癌病程、病理、疗效等临床病理参数的关系；采用RNAi方法稳定抑制肝癌高转移细胞株中SUMO-1基因的表达，通过体外、体内实验来研究对肝癌侵袭与转移的影响，通过RT-PCR、western blot来研究SUMO-1基因表达抑制后与肝癌侵袭与转移相关基因、信号通路的变化；通过蛋白组学方法来发现新的SUMO-1基因调控蛋白，进一步阐明SUMO-1基因和肝癌侵袭与转移的关系。为以SUMO-1基因为靶点诊治肝癌提供实验依据。

Growing evidence shows that SUMO-1 plays a critical role in development of malignant tumor. By means of RT-PCR and western blot we found that SUMO-1 was upregulated in both hepatocellular carcinoma(HCC) specimens and HCC cell lines. Wound healing and Traswell assays revealed that the migration capacity of HCCLM3 was slowed down by transfection of SUMO-1 shRNA.Thus, the results suggest that SUMO-1 is involved in regulation of invasion and metastasis of HCC. Therefore, we will entail RT-PCR, Western blot and Immunohistochemistry to evaluate the relationship between the expression levels of SUMO-1 and clinicopathological grades, and determine the effects of SUMO-1 expression on the response to treatment and on prognosis In addition, SUMO-1 shRNA will be transfected into high metastatic cell lines of HCC and the inhibitory effects of SUMO-1 shRNA on cell migration and metastasis will then be examined in vitro and in vivo. Changes of genes expression in signaling pathways related to invasion and metastasis of HCC will be detected by RT-PCR, western blot. In order to further elucidate the relationship between SUMO-1 gene and HCC invasion and metastasis, proteomic approaches will be applied to identify new proteins regulated by SUMO-1. These findings will help to clarify whether the SUMO-1 gene could serve as a biomarker and target for diagnosis and treatment of HCC.