子宫颈癌是常见的妇科恶性肿瘤，寻求肿瘤超声无创治疗是目前全球研究热点。高危型HPV病毒感染是宫颈癌发生的主要风险，而肿瘤的发生、发展与内质网应激反应有着紧密的联系。目前认为低强度非致死量超声诱导细胞凋亡是超声治疗肿瘤的重要生物学基础之一。研究表明，高危型HPV宫颈癌患者GRP78蛋白的阳性表达率明显增加。但不同HPV亚型宫颈癌细胞对聚焦超声的个体化内质网反应及机制目前尚未见报道。基于这些理论及初步研究结果，可以将GRP78作为高危型HPV宫颈癌超声治疗的一个靶点。本研究以人宫颈癌不同HPV亚型细胞为研究对象，检测不同非致死剂量聚焦超声对不同HPV亚型宫颈癌细胞的影响，了解不同HPV亚型宫颈癌细胞个体化内质网反应。并将GRP78作为靶点蛋白，研究了解内质网途径在聚焦超声诱导细胞凋亡中的调控作用及其机制，为聚焦超声杀伤肿瘤细胞的作用机制研究提供新的线索。

Cervical cancer is one of the mostly common gynecologic malignant tumors.It is very necessary to seek ultrasonic non-invasive treatment for cervical cancer.It is well known that high-risk HPV infection is the main pathogeny,and the occurrence and development of tumor is closely related to endoplasmic reticulum stress.The non-lethal dose of focused ultrasound induced cell apoptosis is one of the important biological basis of ultrasonic treatment for the tumor.Our reserch shows that the positive expression of GRP78 patients increased in high-risk HPV subtype patients.However,the reaction of different HPV subtypes of cervical cancer cells to focused ultrasound and related mechanism have not reported.GRP78 could be a target protein uesed for the ultrasonic treatment of high-risk HPV infection cervical cancer cell.Our research will assess the effect of different dose focused ultrasound on HPV subtypes cervical cancer cell lines.We will measure individual endoplasmic reticulum reaction of different HPV subtypes.Also we plan to assess the regulation effect of endoplasmic reticulum pathway induced by focused ultrasound in cell apoptosis.Our project will provides new clues to study the mechanism of focused ultrasound treated for cervical cancer and explore a novel anti-tumor treatment.