发展一套快速、准确、专一性好的脱毛浸灰程度监测方法是当今制革工业的一项迫切需求。本项目拟结合荧光传感与分子印迹技术构筑一种新的荧光传感器，对皮胶原中的专一性物质（4-羟基-L-脯氨酸，L-Hyp）进行识别，以实现浸灰程度实时监测。首先，通过分子模拟的方法通过分子动力学模拟设计4-羟基-L-脯氨酸分子印迹层；其次，采用ATRP的方法将分子印迹层接枝于磁性碳量子点表面，创制具有高效选择识别性、响应灵敏的核-壳结构4-羟基-L-脯氨酸分子印迹荧光传感器；将得到的探针用于脱毛浸灰模拟物和脱毛浸灰液中目标物质的识别，构筑相应的4-羟基-L-脯氨酸的荧光分析方法，并将目标物质的浓度与皮革的理化性能建立对应关系，进而实现皮革工艺浸灰程度定量监测和监控，为皮革的科学可控加工提供理论依据。

It is an urgent task to develop a fast, reliable and accurate method for liming and unhairing process in leather field. To achieve this aim, a magnetic 4-hydroxyl-L-proline (L-Hyp) imprinted flourescence sensor was constructed. Firstly, the L-Hyp imprinted film was designed by molecular simulation. Subsequently, the optimized composition of pre-polymerization solution was prepared. And the imprinted film was grafted on the surface of carbon dots modified magnetic nanoparticles by atom transfer radical polymerization (ATRP), the as-prepared nano-materials was referred as magnetic L-Hyp imprinted fluorescence sensor. The selectivity and recognition mechanism of sensor were investigated by computational and experimental methods. Finally, the analysis performance for L-Hpy was carried out in liming and unharing liquor, and the physical performance of the obtained embryo leathers was test, to construct the relationship between concentration-signal-performance. To provide the theory basis for scientific controlled and digital processing of leather industry.