课题基金基金详情
间充质干细胞通过调节炎症反应及促进新生物形成治疗盆底器官脱垂的机制研究
结题报告
批准号:
81701422
项目类别:
青年科学基金项目
资助金额:
20.0 万元
负责人:
李春波
依托单位:
学科分类:
H0409.女性生殖系统损伤与修复
结题年份:
2020
批准年份:
2017
项目状态:
已结题
项目参与者:
戴志远、舒慧敏、杜贵强、李春、贾林燕、徐筱倩
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中文摘要
盆底器官脱垂(POP)是妇科疾病的热点和难点之一,目前以补片为基础的手术治疗效果尚不满意,组织工程的应用为POP的治疗提供了新的思路。研究表明间充质干细胞植入机体后,不仅可以促进新生物形成、重建组织缺损,还可以调节炎症反应及疤痕组织增生。本课题拟将腺病毒介导荧光蛋白标记的肌源性干细胞(MDSCs)和脂肪源性干细胞(ADSCs)植入聚乳酸-羟基乙酸共聚物(PLGA)及脱细胞小肠粘膜下层(SIS)补片,成肌诱导后植入大鼠POP模型或皮下,荧光显微镜了解干细胞在体内的转归,组织学及生物力学测试明确组织工程补片的修复情况。ELISA, western blotting, RT-PCR及免疫组化等检测植入物及周围组织中炎症因子的表达及炎症细胞的浸润、增殖情况,明确干细胞对补片诱导的炎症反应的调节作用,并进一步探讨TGF-β/Smad信号通路在干细胞调节成纤维细胞增殖、分化及疤痕组织增生中的作用。
英文摘要
Pelvic organ prolapse (POP) has been one of the hotspots and difficulties of gynecological diseases. The surgical therapeutic efficacy based on a mesh is not so satisfaction. The application of tissue engineering offers a new way for the treatment of POP. Studies showed that mesenchymal stem cells implanted into the body, not only could promote new tissue formation for reconstruction of tissue defects, but also could regulate the inflammatory response and the scar tissue hyperplasia. In the present study, we seeded muscle-derived stem cells (MDSCs) and adipose-derived stem cells (ADSCs) labeled with fluorecent protein mediated by adenovirus into Poly(lactic-co-glycolic acid) (PLGA) synthetic and small intestinal submucosa (SIS) biological meshes. After the compounds were induced myogenic differentiation, they were grafted in a model of POP in rats. After implantation, fluorescence microscope was used to measure the prognosis of stem cells, and histological and biomechanical testing was used to measure the repair procedures of POP. ELISA, western blotting, RT-PCR and immunohistochemistry were used to measure the expression level of inflammatory factors and the proliferative and differentiation of inflammatory cells to definite the function of stem cells in regulating the inflammatory response. We also investigated the function of TGF-β/Smad signaling pathway in modulating the proliferative and differentiation of fibroblast and the role of scar tissue hyperplasia induced by stem cells.
本研究项目的结果包括四个方面内容:1)围绕干细胞促进组织再生及调控炎症反应进行深入研究:通过制备脱细胞羊膜支架,并评估其物化性能,负载ADSCs可以重建损伤的子宫内膜。2)制备多孔的支架材料-SMP,并证实该材料可以促进细胞的增殖;3)围绕新技术腹股沟韧带悬吊术治疗盆底器官脱垂的研究:通过尸体解剖,对相关悬挂点进行测量,定位,并应用于临床实践。4)首次探索宫颈癌类器官的构建,为后续研究奠定基础。
期刊论文列表
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科研奖励列表
会议论文列表
专利列表
DOI:10.1016/j.asjsur.2019.07.014
发表时间:2020
期刊:Asian Journal of Surgery
影响因子:3.5
作者:Chun-bo Li;Ke-qin Hua
通讯作者:Ke-qin Hua
Laparoscopic inguinal ligament suspension with uterine preservation for pelvic organ prolapse: A retrospective cohort study
腹腔镜保留子宫腹股沟韧带悬吊术治疗盆腔器官脱垂:一项回顾性队列研究
DOI:10.1016/j.ijsu.2018.04.020
发表时间:2018-06-01
期刊:INTERNATIONAL JOURNAL OF SURGERY
影响因子:15.3
作者:Li, Chunbo;Shu, Huimin;Dai, Zhiyuan
通讯作者:Dai, Zhiyuan
Laparoscopic inguinal ligament suspension combined with hysterectomy for the treatment of uterovaginal prolapse
腹腔镜腹股沟韧带悬吊联合子宫切除术治疗子宫阴道脱垂
DOI:10.1111/jog.14035
发表时间:2019-09
期刊:Journal of Obstetrics and Gynaecology Research
影响因子:1.6
作者:Li Chunbo;Dai Zhiyuan;Shu Huimin
通讯作者:Shu Huimin
Do Pelvic Organ Prolapse Quantification Examination Ba and D Guide the Selection of Operation for Severe Pelvic Organ Prolapse?
盆腔器官脱垂量化检查Ba和D能否指导重度盆腔器官脱垂手术的选择?
DOI:10.1080/08941939.2018.1533055
发表时间:2020-05
期刊:J Invest Surg
影响因子:--
作者:Chunbo Li;Huimin Shu;Zhiyuan Dai
通讯作者:Zhiyuan Dai
Laparoscopic inguinal ligament suspension versus laparoscopic sacrocolpopexy in the treatment of pelvic organ prolapse: study protocol for a randomized controlled trial.
腹腔镜腹股沟韧带悬吊术与腹腔镜骶骨阴道固定术治疗盆腔器官脱垂:随机对照试验的研究方案
DOI:10.1186/s13063-018-2494-x
发表时间:2018-03-05
期刊:Trials
影响因子:2.5
作者:Li C;Dai Z;Shu H
通讯作者:Shu H
认知训练对成功老龄化影响机制的研究
  • 批准号:
    30770769
  • 项目类别:
    面上项目
  • 资助金额:
    29.0万元
  • 批准年份:
    2007
  • 负责人:
    李春波
  • 依托单位:
焦虑症患者精神病理学机制的功能磁共振成像研究
  • 批准号:
    30200081
  • 项目类别:
    青年科学基金项目
  • 资助金额:
    22.0万元
  • 批准年份:
    2002
  • 负责人:
    李春波
  • 依托单位:
国内基金
海外基金