矿物元素结合肽是一种具有螯合矿物离子能力的活性肽，近来研究表明其不仅能促进矿物质的吸收，还可具有较好的抗氧化特性。本项目拟以罗非鱼肉蛋白酶解物为研究对象，应用超滤、固定化金属螯合亲和层析（IMAC）、凝胶过滤色谱、反相高效液相色谱等技术对罗非鱼肉蛋白酶解物中矿物元素结合肽进行分离、纯化，并研究不同矿物元素（Ca2+、Fe2+、Zn2+）结合肽在抗氧化特性上的差异性。在此基础上研究矿物元素结合肽的一级结构、空间构象和高抗氧化特性之间的内在联系，通过对矿物元素结合肽进行分子修饰进一步验证构效关系，最后研究螯合作用对矿物元素结合肽的空间结构与抗氧化特性的影响，探明螯合物的构效关系，阐明分子机理。本项目可为兼具抗氧化与促矿物吸收的双效功能肽的研究提供理论基础和技术指导，具有重要的理论价值和现实意义。

Mineral-binding peptides could chelate with the mineral ions. Rcently, some results showed that mineral-binding peptides could not merely improve the minerals absorption but also have good antioxidant activity. The objective of this work is to separate and purify calcium-binding peptides, ferrous iron-binding peptides, and zinc-binding peptides from tilapia hydrolysates by using ultrafiltration, immobilized metal affinity chromatography (IMAC), gel filtration chromatography, HPLC, etc and to investigate the differences in the antioxidant properties. Based on our previously work, this work will identify the relationship of primary and spatial structure of mineral-binding peptides and the high antioxidant activity. The probable structure and activity relationship will be verified by molecular modification. Then this work will explore the effects of chelation on the antioxidant and structural properties of mineral-binding peptides. This work will provide the theoretical basis and technical guiding significance for the mineral-binding peptides with high antioxidant properties.