针对目前无抗体胃癌标志物分析体系发展过程中凸显出的抗干扰能力差、特异性低等瓶颈问题，本项目拟以具有高诊断价值的胃癌糖蛋白标志物为模板分子，通过硼酸亲和技术制备胃癌标志物定向磁性表面分子印迹聚合物材料，并应用于实际样品中胃癌标志物的高选择性识别、萃取。以生物相容性、稳定性和易修饰性的Fe3O4@Au纳米粒子为载体，基于硼酸与胃癌标志物糖蛋白糖基之间的高亲和可逆共价键和作用，将模板分子以稳定构象有序、定向固定在Fe3O4@Au纳米粒子表面，再通过苯胺自聚合制备出匹配模板分子大小和形状的3D印迹内腔，实现印迹材料适用宽pH范围、高抗基质干扰和特异性识别胃癌标志物的目标。进一步通过考察印迹材料的吸附容量、吸附特异性和吸附速率，研究其对模板分子的固定、识别和释放机理。本项目研究内容不仅为胃癌标志物的高选择性富集材料发展提供新技术和新思路，更有助于推动无抗体胃癌标志物分析技术更加瞄准解决临床实际问题。

The complex matrix of real samples and the unsatisfactory specificity are two challenges in the antibody-free biomarker determination in the gastric cancer diagnosis. In this project, four glycoprotein biomarkers of the gastric cancer will be selected as the template molecules, and Fe3O4@Au nanoparticles will be treated as the magnetic carrier. The template molecules will be oriented immobilized on the surface of the Fe3O4@Au nanoparticles through the unique reversible covalent binding affinity between the boronate and the glycoprotein biomarkers of the gastric cancer. On this basis, oriented magnetic molecularly imprinted polymers will be prepared by surface imprinting for the selective recognition of the gastric markers in the real samples. The mechanism investigation on the fabrication and the recognition of the imprinted materials will be studied, and the specificity of the imprinted materials will be compared with the traditional non-oriented imprinted materials. The magnetic molecular imprinting materials prepared in this project would favor the high selectivity towards the gastric markers, and exhibit superb tolerance for interference. This project will not only has significant scientific values for the development of high selective enrichment of the gastric tumor biomarkers, but also would show its promising potentials to promote the antibody-free analysis of gastric markers towards clinical applications.