肌内脂肪(IMF)含量是影响猪肉品质的最重要因素，在基因水平研究肌内脂肪生成的调控机理是改善猪肉品质的根本途径之一。人、鼠上研究发现，MSTN对调节IMF沉积起重要作用。然而，MSTN在调控猪IMF沉积中的作用还不清楚。我们前期发现，miR-130b可减少梅山猪皮下脂肪沉积，并且通过生物信息学软件预测显示miR-130b与MSTN之间具有很强的靶向关系。然而，miR-130b能否靶向MSTN调控梅山猪IMF沉积还需要进一步研究。本研究首先通过双荧光素酶报告基因系统、“过表达”和“干扰”等方法确认miR-130b与MSTN之间靶向关系，然后以肌内脂肪细胞为模型，重点研究miR-130b和miR-130b-MV介导MSTN对猪IMF沉积的调节作用。通过本研究可明确miR-130b所介导的MSTN对猪IMF沉积的调节作用，为进一步提高猪肉中IMF含量进而改善猪肉品质提供理论依据。

With the improvement of people's living standard, the pork quality has aroused wide attention of producers and consumers, and the concent of intramuscular fat (IMF) is the most important factor that influences the quality of pork. Research of the regulation mechanism of pig intramuscular fat deposition is one of the fundamental way to improve the pork quality. Recent studies have found that MSTN (myostatin), a negative regulatory factor of skeletal muscle growth, plays an important role in intramuscular fat deposition. However, whether miRNAs can regulate pig IMF deposition through MSTN is still unknown. .In our previous study, we found that miR-130b can reduce subcutaneous fat deposition of pigs by inhibiting its target gene ppar-gamma expression, we also found that miR-130b was one of the strong target of MSTN through bioinformatics software prediction. However, whether miR-130b can regulate MSTN at the post-transcription level and affect intramuscular fat deposition of pigs still needs further study. Our present study aims to：(1)clarify the special roles of MSTN in the porcine intramuscular fat deposition and explore the possible post-transcription mechanism mediated by miR-130b, (2) explore the regulation role of MV delieved miR-130b (miR-130b-MV) in MSTN expression and demonstrate the effect of miR-130b-MV in the regulation of porcine intramuscular fat deposition by targeting MSTN in in vivo and in vitro models..Through our present study, we can further know the special role of MSTN in regulating the pig intramuscular fat deposition and the post-transcription mechanism mediated by miR-130b, secondly, we can discuss whether MV can be used as efficient delivery vehicle of miR-130b. Our results will further improve the IMF content in pork and provide certain theoretical basis for improving the quality of pork.