目前有关氮杂环多羧酸参与构建多钨氧酸盐过渡–稀土异金属簇聚物和框架材料报道很少，还没有关于多钨氧酸盐过渡–稀土异金属材料作为电极修饰材料进行电化学生物传感性能研究的报道。本项目拟利用水热条件下原位形成的过渡金属嵌入多钨氧酸盐构筑块与稀土离子和氮杂环多羧酸配体相结合的反应策略和以已制备的过渡金属嵌入多钨氧酸盐前驱体与稀土离子和氮杂环多羧酸配体在混合溶剂中分步组装策略来合成氮杂环多羧酸参与构建多钨氧酸盐过渡–稀土异金属簇聚物和框架材料，找到形成目标材料的关键控制因素和反应规律。利用目标材料来构筑电化学生物传感器，详细考察其稳定性、重复性、灵敏度及在复杂环境中选择性，探索目标材料结构改变对构筑的电化学生物传感器的性能变化的影响规律，阐明构筑的电化学生物传感器对待测生物组分的检测识别机理。该项研究为实现目标材料构筑的生物传感器在生物检测、癌细胞标记物检测等领域的开发与应用奠定基础。

To date, the relevant reports on nitrogen heterocyclic polycarboxylic acid participating polyoxotungstate-based transition-metal-rare-earth heterometallic aggregates and framework materials are very rare and there is no report on electrochemical biosensoring properties involving polyoxotungstate-based transition-metal-rare-earth heterometallic materials as electrode modified materials. In this project, we will plan to utilize the one-pot reaction strategy of combining in-situ formed transition-metal incorporated polyoxotungstate buliding blocks with rare-earth ions and nitrogen heterocyclic polycarboxylic acid ligands under hydrothermal conditions and the step-by-step assembly strategy of reacting the prefabricated transition-metal incorporated polyoxotungstate precursors with rare-earth ions and nitrogen heterocyclic polycarboxylic acid ligands in mixed solvents to prepare novel nitrogen heterocyclic polycarboxylic acid participating polyoxotungstate-based transition-metal-rare-earth heterometallic aggregates and framework materials and find out the key controlling factors and reaction rules of forming target materials. Also, we will use the target materials to construct electrochemical biosensors, systematically investigate their stability, repeatability, sensitivity and selectivity in complex environments, explore the influence laws of structural changes of target materials on the performances of the as-prepared electrochemical biosensors, and illuminate the detection and recognition mechanisms of the as-prepared electrochemical biosensors for the detecting biological components. As a result, this research will lay the foundation for realizing the developments and applications of the biosensors constructed from target materials in the fields of biological detection, cancer cell marker detection, etc.