前列腺素转运载体MRP4与PGT在斑马鱼排卵中的功能研究

排卵是指成熟卵子从滤泡中释放出来的动态过程，受到复杂而精准的调控。前列腺素（Prostaglandin，PG）是调控排卵的关键因子，其很难自由通过生物膜，需要依赖前列腺素转运载体的运输。然而，有关前列腺素转运载体在哺乳动物排卵中的功能研究只有零星报道，在鱼类排卵中的研究更是一片空白。我们初步研究发现，前列腺素转运载体中负责PG外流的MRP4(Multidrug Resistance Protein 4 )与负责PG摄入的PGT (Prostaglandin Transporter)可能参与调控斑马鱼的排卵活动。鉴于此，本项目拟以斑马鱼为模型，选择MRP4与PGT为切入点，采用抑制剂处理、基因敲除及辅以PG补偿的方法，研究前列腺素转运载体对斑马鱼排卵过程中滤泡细胞内外PG浓度的调控以及对排卵的影响。本项目的研究将揭示前列腺素转运载体在鱼类排卵中的功能，也为其他脊椎动物的排卵调控研究提供参考。

Ovulation is a complex and precise biological process during which mature oocytes are released from the surrounding follicle wall into the ovarian cavity. It is well established that the PGs is one of the key fact during the ovulation. While it is noteworthy that PGs are diffuse poorly across model biological membranes and need relies on prostaglandin transporter. However, it have sporadic relate study about the function of prostaglandin transporter on ovulation in mammals. And even no study about the function of prostaglandin transporter on ovulation in fish. Our preliminary data suggest that MRP4 (responsible for PGs efflux) and PGT (responsible for PGs influx) are involved in regulate ovulation in zebrafish. Therefore, in this project, using zebrafish as the experimental model, two important prostaglandin transporters, including MRP4and PGT are selected，utilize inhibitor treatment, gene knockout and PGs replacement to investigate the roles of prostaglandin transporter on regulate the concentration between extracellular and intracellular and regulate the ovulation. The implementation of this project will reveal the function of MRP4 and PGT on ovulation in fish, and lead us to a better understanding of the regulation of ovulation in vertebrate.