有序的滋养细胞增殖、分化进程在胚胎植入、胎盘形成及妊娠维持中起着重要的作用。胚胎着床失败及早期妊娠丢失等与滋养细胞增殖和分化不良密切相关。我们前期蛋白质组学研究发现，半乳糖凝集素-1（Galectin-1）在人EPL滋养细胞中表达较正常对照显著降低，提示Galectin-1在早期胎盘发育、母-胎对话中扮演了重要角色。本项目拟应用小鼠滋养层干细胞增殖-分化模型模拟滋养层细胞发育进程，并结合体外基因表达调控技术、胚胎显微操作及相关动物模型，完整地揭示Galectin-1在滋养细胞增殖-分化进程中表达的时空特征、调控模式及相关重要分子间作用方式，并应用小鼠动物模型观察Galectin-1在胚胎着床和早期妊娠维持中的作用，为揭示着床失败和早期妊娠丢失机制、寻求相关治疗方法提供有价值的理论依据。

Trophoblastic proliferation and differentiation in an orderly manner play a critical role in the embryonic implantation, placentation and maintencance of early pregnancy. The embryo implantation failure and early pregnancy loss are intensively related with poorly trophoblastic proliferation and differentiation. Our previous proteomics study on human EPL chorionic tissues found that galectin-1 was downregulated significantly in trophoblastic cells of EPL when compared with the controls, which suggestting that galectine-1 has an important role in early placental development and maintenance of pregnancy. In this protocol, we aim to reveal the characteristics of spatial-temporal expression and epigenetic regulative pattern of galectin-1 and related molecules during the development of trophoblastic cells, placentation and early pregnancy by using mouse trophoblast stem cells as a proliferation-differentiation model , combination with the in vitro gene control techniques, embryonic micromanipulation, pseudo-pregnancy mouse model and stress-induced mouse models. Ultimately, we aim to determinate the pathologic mechanism of EPL, and provide valuable information and seek possible direction for cellular and molecular treatment.ferentiation model stimulation the development process of trophoblast cell, combination with the in vitro gene expression and regulation, embryo micromanipulation and related animal models, complete reveal of galectin-1 characteristics of spatial-temporal expression, expression regulation patterns, and related important molecular mode of action in trophoblastic proliferation/differentiation. With a mouse model, investigate the role of galectine-1 in embryo implantation and early pregnancy maintenance. Ultimately reveals the mechanisms of embryo implantation and EPL, provide valuable basis for seeking treatment methods.