调理脾胃针法是治疗糖尿病肾病（DN）有效的针法。前期研究结果已证实其可有效改善DN的临床症状和体征，降低蛋白尿水平，但机制尚未阐明。足细胞损伤是DN发生发展的重要因素。足细胞上皮-间充质转分化（EMT）的过程概括了DN早期足细胞损伤的绝大部分特征，影响或逆转EMT成为了近期的研究热点。申请人前期研究发现针刺可以改善足细胞损伤，降低肾组织二肽基肽酶4（DPP4）的表达。基于现代研究中DPP4/SDF-1a/TGF-b轴在介导EMT中扮演的重要角色，我们提出“调理脾胃针法通过DPP4/SDF-1a/TGF-b信号轴影响足细胞EMT降低DN蛋白尿”的中心假说。拟建立针刺2型DN大鼠效应平台，以“调理脾胃针法”为干预手段，检测蛋白尿等效应指标，观察肾小球结构及足细胞超微结构的改变，通过足细胞EMT损伤标识物与DPP4/SDF-1a/TGF-b轴蛋白的表达与针刺作用相互之间联系，阐释针刺作用机制。

The Tiaolipiwei acupuncture method is a therapy with unique characteristics of traditional Chinese medicine, which is easy and effective. Previous studies have confirmed that it can effectively improve the clinical symptoms and signs of type 2 diabetic nephropathy, reduce urine protein levels, regulate oxidative stress levels and immune environment. Animal experiments show protective effects on podocyte injury. However, the mechanism under it has not been fully elucidated, which has affected the application and promotion of the therapy. Therefore, elucidating the scientific mechanism of Tiaolipiwei acupuncture method will help to expand its clinical application in type 2 diabetic nephropathy and prevent the development of end-stage renal disease. This study intends to establish an effective platform of type 2 diabetic nephropathy rat. Using the Tiaolipiwei acupuncture method as an intervention, we try to detect proteinuria and other effect indicators, observe the glomerular structure and the slight structural changes of podocytes, interpret the intrinsic relationship between acupuncture and the expression of podocyte injury markers, EMT markers, and DPP4/SDF-1a/TGF-b. In those conditions, the study aims to indicate that Tiaolipiwei acupuncture treat type 2 diabetic nephropathy by regulating DPP4/SDF-1a/TGF-b pathway to improve the EMT level of podocytes and reduce proteinuria, thus providing a new reference for the study of the mechanism of acupuncture treatment of glycosaminoglycan.