棕点石斑鱼是我国南方主要海水养殖品种之一。近年来部分养殖户为了追求经济利益，过量投喂高脂饲料，导致鱼体肠炎与脂肪肝病并发，免疫力下降。因而更易受外源致病因子感染而爆发疾病。中草药因其绿色环保、无耐药性等优点，在鱼病防治中得到日益广泛的应用。哺乳动物研究发现，黄柏具有消炎，降脂，抗脂肪肝的功效，然而，黄柏对鱼类的作用机理尚不明确。本项目拟通过体内投喂实验，在建立棕点石斑鱼脂肪肝模型的基础上，检测黄柏及其主要成分小檗碱对高脂饮食棕点石斑鱼生长、血清/肝脏生理生化指标的影响；并通过转录组测序分析，挖掘高脂饮食以及黄柏/小檗碱作用下高脂饮食棕点石斑鱼头肾/肝脏/肠道中特异性表达的免疫、脂代谢基因/通路。本研究不仅为系统揭示黄柏对高脂饮食棕点石斑鱼的免疫/血脂代谢调控机制奠定基础，为黄柏应用于防治鱼类脂肪肝病提供理论依据，对推动渔用中草药的开发应用和棕点石斑鱼健康养殖的可持续发展具有重大意义。

Epinephelus fuscoguttatus is one of the major mari-culture species developed in the Southern provinces in recent years. However, enteritis and fatty liver diseases developed when farmers over fed fish with high lipid diet to reduce cost. Diseased fish were more prone to pathogens, and mortaliy increased sharply.Chinese medicinal herbs were more and more popular in the disease control practices of fish farming.They were environmental friendly and produce no drug-resistance.Studies demostrated that Phellodendron amurense has anti-inflammatory, blood lipids lowing, and anti-fatty liver effects in mammals. However, its effects in fish were not clear yet. This project firstly aim to establish a fatty liver disease model in E. fuscoguttatus based on in vivo feeding experiments. Then the effects of Phellodendron amurense and its major content-berberine on the growth and physiological biochemistry parameters of E. fuscoguttatus fed with high lipid diet were examined.Lastly, specific immune and lipid matabolism related genes/pathways differentially expressed under the effect of Phellodendron amurense/berberine in the headkidney,liver and intestine of E. fuscoguttatus fed with high lipid diet were identified by transcriptome sequencing and analysing techniques. The results of this study will not only be helpful to develop safe and efficient drugs for controlling the enteritis and fatty liver diseases in E.fuscoguttatus, but also be helpful to reveal the mechanism of Phellodendron amurense/berberine’s regulatory effects on the immune and blood lipid metabolism systems in E.fuscoguttatus. Therefore, this study will play an important role in promoting the development and application of Chinese herbal medicines for fish and the sustainable development of healthy culture of E. fuscoguttatus.