针对中药方剂药效物质基础难以阐明的科学问题，为了解析中药方剂“多成分-多靶点”的作用特点，本课题拟建立一种新型的多靶标脉冲超滤-质谱在线联用技术，该技术将脉冲超滤单元进行微量化处理，并且将多个脉冲超滤单元进行在线并联，同步实现“多靶标-多成分”的快速发现。本项目选取临床使用频率较高，但药效物质不明的中药降血糖制剂——参芪降糖颗粒为传统代表性方剂，以α-葡萄糖苷酶、醛糖还原酶、PTP1B和PPARγ等多个临床确证有效的降糖靶点作为活性指标，基于多靶标脉冲超滤质谱技术，快速发现参芪降糖颗粒中的活性成分（群），明确活性小分子与各靶标之间的相互作用，在此基础上，对参芪降糖颗粒的活性成分（群）进行体内代谢规律研究，监测化学成分在体内的变化，从体内和体外两方面阐明其药效物质基础。本项目的研究结果将为中药方剂的药效物质基础研究探索一种新思路。

For the scientific issues that it’s difficult to elucidate the bioactive constituents in Chinese herbal formula, and in order to resolve the proprietary of “multi-component, multi-target”, a novel online multi-target pulse ultrafiltration mass spectrometry technology is established. This technology possesses trace pulses ultrafiltration units. Multi active components could be quickly discovered by connecting a plurality of parallel multi-pulses ultrafiltration units. Shenqi Jiangtang Granule (SJG) is a commonly used TCM preparation, but the active components are not clear, which is selected as a representative prescription. Multiple clinical validated hypoglycemic targets are chose as activity index, such as α-glucosidase, aldose reductase, PTP1B and PPARγ, etc. The bioactive constituents in SJG are rapid discovered, and the interaction between the active components and the target proteins are clarified based on the multi-target pulse ultrafiltration mass spectrometry technology. Then, metabolic study of the active components in SJG is implemented through monitoring the changes of chemical constituents in vivo and in vitro. The results of this project will provide a methodology demonstration for the basic research of elucidating the bioactive constituents in Chinese herbal formula.