Quality evaluation of triterpenoids in Ganoderma and related species by the quantitative analysis of multi-components by single marker method
Quality evaluation of triterpenoids in Ganoderma and related species by the quantitative analysis of multi-components by single marker method
复制标题
单标记法多成分定量分析灵芝及相关品种三萜类化合物的质量评价
DOI：
10.1080/10826076.2018.1531292
复制
发表时间：
2018-08
影响因子：
1.3
通讯作者：
Jizhong Yan
中科院分区：
文献类型：
--
作者：
Hui Zhang;Huijie Jiang;Yan Chen;Jianan Wang;Jizhong Yan
摘要目的：建立灵芝及其近缘种三萜类化合物的多组分单标记定量分析方法。方法：采用高效液相色谱法和定量分析法测定了10种厚朴三萜类化合物的含量。在QAMS的基础上，采用主成分分析（PCA）和聚类分析（CA）对灵芝样品进行了鉴别。结果：10种类型的挥发性三萜类化合物线性关系良好（R2 ≥ 0.9992）。灵芝酸C、灵芝酸LM 1、灵芝酸G、灵芝酸B、灵芝酸A、灵芝烯酸D、灵芝酸D、灵芝酸D和灵芝酸F相对于灵芝酸A的相对校正因子（RCF）分别为0.929、1.011、0.608、1.041、0.997、2.464、0.891、分别为0.826和0.722。采用QAMS法测定了29批灵芝及其近缘种中的10种有效成分。通过PCA和CA将19批含阿魏酸A的样品大致分为三类。结论：所建立的QAMS方法与主成分分析和元胞自动机相结合，为灵芝药材的快速评价和真伪鉴别提供了一种准确的方法。图形摘要
Abstract Objective: To establish a quantitative analysis of multi-components by single marker (QAMS) method for the determination of triterpenoids and distinction of Ganoderma and related species. Methods: Ten ganoderma triterpenoids were determined by HPLC in addition to a QAMS method. On the basis of QAMS, principal component analysis (PCA) and cluster analysis (CA) were used to distinguish Ganoderma samples. Results: Ten types of ganoderma triterpenoids had good linearity of regression coefficients (R2 ≥ 0.9992). Relative correction factors (RCFs) of lucideric acid C, lucideric acid LM1, ganoderic acid G, lucideric acid B, lucideric acid A, ganoderenic acid D, ganoderic acid D, lucideric acid D and ganoderic acid F with reference to ganoderic acid A were 0.929, 1.011, 0.608, 1.041, 0.997, 2.464, 0.891, 0.826 and 0.722, respectively. Ten types of ganoderma triterpenoids in twenty-nine batches of Ganoderma and related species were determined by QAMS. Nineteen batches of samples containing ganoderma acid A were roughly divided into three categories by PCA and CA. Conclusion: The developed QAMS method, in combination with PCA and CA, served as an accurate method for the rapid evaluation and authenticity identification of Ganoderma. GRAPHICAL ABSTRACT
登录
查看更多内容
影响因子：
2.6
作者：
Jiang Y;Chen H;Wang L;Zou J;Zheng X;Liu Z
通讯作者：
Liu Z
DOI：
--
发表时间：
2009
期刊：
The Chinese Pharmaceutical Journal
影响因子：
--
作者：
Su Jian-fen
通讯作者：
Su Jian-fen
DOI：
10.1080/10826076.2016.1169429
发表时间：
2016-05-08
影响因子：
1.3
作者：
Wang LL;Zhang YB;Sun XY;Chen SQ
通讯作者：
Chen SQ
DOI：
10.4268/cjcmm20120209
发表时间：
2012
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
Ye Li;Zhongmin Zhu;Weixi Yao;Ruo-yun Chen
通讯作者：
Ye Li;Zhongmin Zhu;Weixi Yao;Ruo-yun Chen
影响因子：
3.4
作者：
Liu, Li-Ying;Chen, Hui;Chen, Ruo-Yun
通讯作者：
Chen, Ruo-Yun