便秘与衰老的相关性研究及温阳健脾法之干预作用

As the population ages, aging problem is becoming more and more serious, and the research on the aging has become an important subject of life science. As a common gien, constipation can influence of the body function , and produce all kinds of diseases, accelerating aging. As is known to all, there is an identify at all times and in all over the world with that constipation can cause aging, but the scientific relationship between them has not confirmed yet. So it's an important significance on exploring the relationship between Constipation and aging, as well as the intervention role of the traditional chinese medical. In this search, we make the animal constipation models with the methods of using laxatives and diphenoxylatum compound, and make animal aging models with the methods of D-Galactopyranose subcutaneous injection. Then we detect the aging index and constipation index, including thymus index, spleen index, SOD activity,LPO content,liver Ca2+-ATP Enzyme activity, MDA content and liquidity of the liver mitochondrial membrane, and detect klotho protein with immunohistochemistry, meanwhile detecting expression level.of klotho protein with the method of Western Blot. Analysing the similarities ,differences and the relationship between the three groups ,we observe the intervention role of the method spleen warming(温阳健脾法) to constipation and aging after treatment. It will be make contribution to further exploration on aging mystery and anti-aging effect of traditional chinese medicine.

随着人口老龄化，衰老问题越来越突出，对衰老的研究一直是生命科学领域重要的课题之一。便秘是一种常见的老年病，可影响机体的功能而变生诸病,进而加速衰老。众所周知，便秘易致衰老的说法古今中外均有认同，但其科学关系尚未证实，因此，探讨便秘与衰老的关系以及中医的干预作用具有重要的意义。本课题通过运用口服泻药法和口服复方苯乙哌啶法复制便秘动物模型、运用D-半乳糖皮下注射法复制衰老动物模型，检测其衰老指标和便秘指标，包括胸腺指数和脾脏指数、测定超氧化物歧化酶(SOD)活性和血清过氧化脂质(LPO)含量，肝组织Ca2+-ATP酶活性, 肝线粒体丙二醛(MDA)含量、肝线粒体膜流动性。免疫组织化学检测klotho蛋白、Western blot法检测klotho蛋白表达水平。分析研究三组之间的异同及相关性，然后采用温阳健脾法治疗，观察其对便秘及衰老的干预作用，为进一步探索衰老之迷及中医抗衰老作用做出贡献。

随着人口老龄化，衰老问题越来越突出，对衰老的研究一直是生命科学领域重要的课题之一。便秘是一种常见的老年病，可影响机体的功能而变生诸病，进而加速衰老。众所周知，便秘易致衰老的说法古今中外均有所述，但其科学关系尚未证实，因此，探讨便秘与衰老的关系以及中医的干预作用具有重要的意义。本课题通过运用口服泻药法和口服复方苯乙哌啶法复制便秘动物模型、运用D-半乳糖皮下注射法复制衰老动物模型，观察三组动物及正常动物某些衰老相关指标和便秘相关指标，来研究探讨便秘与衰老的相关性，并用中医温阳健脾的药物分大、中和小剂量治疗，且与西药莫沙必利对照，观察其治疗便秘和抗衰老的作用。.研究结果表明三组大鼠粪便形态，粪便重量及数量（粒数）、肠道传输功能等均有便秘改变，结肠组织中SCF、c-Kit蛋白的表达下降，结肠壁VIP、SP、5-HT、Ach浓度下降，NO升高，均符合便秘表现。其胸腺指数下降，血清超氧化物歧化酶(SOD)浓度下降、过氧化脂质(LPO)含量升高、丙二醛（MDA）升高、谷胱甘肽过氧化物酶（GSH-PX）降低，对血清溶血素水平（IGg）、血糖和总胆固醇无明显变化。经治疗后，以上模型组出现变化的指标均可出现改善，其中温阳健脾方大剂量组疗效最佳，略优于莫沙必利阳性对照组。.分析结果得出，三组模型便秘相关指标和衰老相关指标均表现出比较一致的趋向，进一步说明便秘和衰老具有一定的联系，便秘可能促进了大鼠衰老的发生，而衰老的大鼠大或伴有一定程度的便秘。通过温阳健脾药物的干预作用，结果说明本方可以改善便秘大鼠的便秘症状，并能够改善大鼠衰老模型的某些衰老相关指标，提示温阳健脾中药具有抗衰老作用。.便秘是一个老年人常见病证，而衰老是人类的必然结果，两者的发生有一定的联系，改善症状，延缓衰老，是人们追求的不懈目标，中医药防治具有广阔前景，尚需进一步研究。

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