膀胱癌具有术后极易复发的特点，其主要原因是术中肉眼难以精准识别微小肿瘤灶及区分肿瘤边界所导致的肿瘤残余，因此术中彻底切除肿瘤组织是降低膀胱癌复发的关键因素。本项目拟在前期工作的基础上以功能性多肽为主体进行分子设计，通过对不同功能的多肽进行稳定性优化和可控性修饰，构建在膀胱癌病灶原位发生响应性自组装的新策略，解决传统分子探针在病灶原位难以长效滞留的瓶颈问题。同时利用该策略的高特异性及高灵敏性特点，通过膀胱腔内灌注的方式对人离体荷瘤全膀胱进行肿瘤的稳定、精准可视化成像，以此实现术中导航下的肿瘤彻底切除，旨在降低膀胱癌的复发。本项目的开展不仅发展了活体原位自组装的新方法，还加速了活体自组装纳米技术在膀胱癌术中导航应用中的临床转化进程，同时为精准检出微小肿瘤灶及肿瘤边界提供了全新的策略。

Bladder cancer has the characteristics of easy recurrence after surgery. The main reason is that it is difficult for the naked eye to accurately identify the microscopic tumor focus and distinguish the tumor boundaries. Therefore, the complete removal of tumor tissue during surgery is a key factor to reduce the recurrence of bladder cancer. Based on the preliminary work, this project intends to design molecular molecules based on functional peptides. By optimizing the stability and controllability of peptides with different functions, the responsive self-assembly of bladder cancer lesions are in situ constructed which overcome the long-term retention of traditional peptide probes in the lesions. At the same time, owing to the high specificity and high sensitivity characteristics of the strategy, the tumor is stably and accurately visualized by intravesical instillation of the human whole bladder, which can guide to complete resection of the tumor under surgery to reduce the recurrence of bladder cancer. The development of this project not only develop the new method of in situ self-assembly but also accelerate the clinical translation process of in vivo self-assembly nanotechnology in the application of bladder cancer surgery, meanwhile provides a new strategy for accurately detecting microscopic tumor lesions and tumor boundaries.