巴泰病毒通过网格蛋白介导的内吞途径入侵N2a细胞机制研究

近年，新发和再发人兽共患病频发，对人和家畜生命造成严重威胁。其中巴泰病毒能引起人非特异性发热,甚至发展为无菌性脑膜炎，家畜出现神经症状甚至引起流产。2012年我们在内蒙地区发热伴有神经症状牛的血清中分离到该病毒，系我国首次分离的牛源巴泰病毒。而目前国内外对巴泰病毒的研究较少，病毒的感染过程尤其是病毒入侵神经细胞的途径尚未明确。因此，我们初步开展BATV通过网格蛋白介导的内吞途径入侵神经细胞的研究。首先利用网格蛋白组装抑制剂、动力蛋白抑制剂及shRNA技术，对网格蛋白及动力蛋白表达进行抑制，干扰网格蛋白介导的内吞途径，分析其表达量下降对BATV入侵细胞效率的影响。再利用内体酸化抑制剂NH4Cl及shRNA技术，下调初级内体Rab5、次级内体Rab7和Rab9，进一步分析内体对BATV入侵细胞效率的影响。该项目对BATV入侵神经细胞机制的研究，为抗病毒策略的制定、抗病毒药物的研发提供依据。

In recent years, emerging and re-emerging infectious diseases including Batai virus disease have threated seriously to human and animal. And Batai virus transmitted to humans and livestock by mosquitoes, which mainly resulted in non-specific fever, even aseptic meningitis in human. And livestock showed abortion and neurological symptoms. In 2012, we have isolated a novel BATV NM/12 strain from bovine serum collected in Inner Mongolia, which was firstly reported in China. Because the lack of research on Batai virus, the process of virus infection is not clear in cell, and the mechanism of BATV entry N2a cell is still unknown. Therefore, we study on the BATV entry into N2a cells by clathrin-mediated endocytosis. CPZ, Dynasore and shRNA were used to disturb the clathrin-mediated endocytosis. Based on the results of such experiments, we will analysis the impact of BATV infected cells by using NH4Cl and down-regulation of Rab5，Rab7 and Rab9. This research will provide new information for understanding the BATV infection. The research of BATV intrusion mechanism can provide the basis for the development of antiviral strategy and antiviral drugs.