乳腺癌是一种侵润性高,容易发生转移的恶性肿瘤，约70%的乳腺癌病人晚期会转移到骨。在乳腺癌中由于雌激素受体的缺失，抑制KISS1/GPR54表达的机制被解除，KISS1/GPR54的上调与其恶性程度及不良预后正相关。骨转移是乳腺癌病人的主要致残和死亡原因，而KISS1/GPR54与乳腺癌骨转移的关系目前尚未有报道。乳腺癌细胞转移到骨微环境后，利用正常骨代谢促进自身的增殖。课题组前期研究结果显示KISS1/GPR54在骨代谢中发挥重要作用；本项目拟通过检测临床标本中KISS1/GPR54的表达，明确其与乳腺癌骨转移的关系，为乳腺癌骨转移的诊断提供新的分子标记；进一步研究骨微环境来源的KISS1吸引高表达GPR54的乳腺癌细胞到骨微环境中的作用，乳腺癌细胞分泌的KISS1对正常骨代谢的影响，明确KISS1/GPR54在乳腺癌骨转移及骨破坏中的作用及机制，寻找治疗乳腺癌骨转移及骨破坏的药物靶点。

Breast cancer is considered a malignant tumour because it is highly invasive and prone to metastasis . Bone metastases are present in 70% of patients with advanced breast cancer .Due to the deletion of estrogen receptor,the inhibiton of KISS1/GPR54 is unloaded in breast cancer.The expression level of KISS1/GPR54 is positively related to the degree of maliganacy and poor prognosis.Bone metastases is the mainly reason that caused the death of breast cancer patients, But the relationship between KISS1/GPR54 and bone metastases is not reported .When breat cancer cell metastasis to bone,normal bone metabolism is uitilized by tumour cell to promote its proliferation.Our data predicted that Kiss1/Gpr54 play important role in bone metabolism. This project intends to unclear the relationship between KISS1/GPR54 and breast cancer bone metastases by a large number of clinical specimens,which provides new molecular markers for the diagnosis of bone metastases .We will further explore the effect of KISS1 from bone mircroenvironment on the attaction of breast cancer metastases to bone ,and the effect KISS1 from breast cancer on the normal bone metabolism.If we figure out the role and molecular mechanism of KISS1/GPR54 in breast cancer bone metastases,it will provide a theoretical basis for treatment of breast cancer bone metastases and bone destruction .