骨质疏松症所伴发的骨折、骨缺损是亟待解决的医学难题。骨质疏松症自体脂肪干细胞（OP-ASCs）在治疗骨质疏松性骨折和骨缺损中具有良好的应用前景。Wnt信号通路在调控干细胞定向分化及骨形成中具有重要作用。我们前期研究显示：激活Wnt/β-catenin信号通路可以促使OP-ASCs的β-catenin从胞质转入胞核，促进成骨转录因子Runx2表达上调。因此我们推测Wnt/β-catenin信号通路可能在OP-ASCs骨向分化中发挥了关键调控作用。本课题拟从不同分子水平干预Wnt信号通路的活化水平，检测该信号通路相关信号分子和骨向分化标志物的表达，筛选调控OP-ASCs骨向分化的关键信号因子，阐明Wnt信号通路在OP-ASCs骨向分化过程中发挥的作用及其分子机制，在此基础上进行靶向调控，恢复及促进OP-ASCs的骨向分化能力，为促进骨质疏松性骨折愈合和骨再生提供新的思路和方法。

The bone defects and fractures associated with osteoporosis are medical problems to be solved. Adipose-derived stem cells (ASCs) from osteoporosis (OP-ASCs) can be a valuable tool for use in osteoporosis therapy. Wnt signaling pathways have been the focus of intense research activity in bone biology laboratories because of their importance in skeletal development, bone mass maintenance, and the regulation of lineage-specific differentiation of stem cells. Our recent studies showed that activation of Wnt/β-catenin signaling pathway can promote nuclear translocation of β-catenin from the cytoplasm, and upregulates expression levels of Runx2. Therefore, we speculate Wnt/β-catenin signaling pathway may play a key role in osteogenic differentiation of OP-ASCs. In this study, in order to clarify molecular mechanism of Wnt signaling pathway regulating osteogenic differentiation of OP-ASCs, we detect signal molecules related to the signal pathway and the expression of specific osteogenic-differentiated markers, and screen the key factors of regulating osteogenic differentiation of OP-ASCs when interventing Wnt signaling pathway. Moreover, the osteogenic differentiation capacity of OP-ASCs is restored and promoted by modifying this signaling pathway. This new idea and methods will provide a effective therapeutic option for healing osteoporotic fractures and promoting bone regeneration.