早期防治肾性骨病，可以降低由此带来的骨折等并发症，提高患者生活质量，但临床上缺乏安全有效的药物。文献及我们前期研究表明β-Catenin在骨形成过程中起着重要的调控作用，健腰密骨片及其有效组分（蛇床子素、补骨脂素、淫羊藿苷）对骨质疏松具有很好的疗效。肾性骨营养不良的早期骨形成降低，但与β-Catenin的关系还不清楚。鉴于此我们提出“健腰密骨片及其有效组分激活β-Catenin信号通路防治肾性骨营养不良”的假说。观察肾性骨营养不良早期β-Catenin的表达；通过构建Ostrix-Cre;Axin1flox/flox小鼠，特异性在成骨细胞敲除Axin1造成过表达β-Catenin，复合5/6肾切除后观察该小鼠的骨丢失情况；健腰密骨片及蛇床子素、补骨脂素、淫羊藿苷对5/6肾切除小鼠干预，是否通过激活β-Catenin信号通路，促进骨形成。从“解剖肾”的角度丰富和发展“肾主骨”理论的科学内涵。

Early prevention and treatment of renal osteodystrophy (ROD) can decrease the incidence rates of complications such as fracture, improve life quality of patients. Our previous research revealed that β-Catenin expression played important roles in regulation of bone formation. Jian Yao Mi Gu Pian and its components osthole, psoralen, icariin are used clinically for primary osteoporosis, and have been proved to be very effective. Recent studies have revealed that bone formation decreased at early stage of ROD, however, its relationship with β-Catenin is still not clear. Therefore, based on our preliminary data, our hypothesis is Jian Yao Mi Gu Pian and its the effective components can prevent and treat ROD through activation of β-Catenin signaling pathway. Our study is to observe the expression of β-Catenin at early stage of ROD. The mouse model of Ostrix-Cre/Axin1flox/flox will be constructed. When Axin1 is specifically knockout in osteoblasts,β-Catenin will be overexpressed. Bone loss will be observed when 5/6 kidney of mouse is resected. Jian Yao Mi Gu Pian and its components osthole, psoralen, icariin will intervent the resected 5/6 kidney of mouse and we will observe whether Jian Yao Mi Gu Pian and its the effective components can promote bone formation through regulation of β-Catenin signaling pathway. Scientific connotation of theory of “kidney controlling bones” will be enrichment and development by the view of “anatomy kidney”.