基于重测序进行全基因组关联分析挖掘大麻CBD含量候选基因

大麻二酚（CBD,cannabidiol）是大麻植物中特有的一类次生代谢产物，具有重要的药用价值。目前，CBD含量的遗传机制尚不明确。挖掘CBD 含量候选基因，是大麻CBD含量分子育种以及探析其遗传机制的基础。我们前期对115份种质资源组成的自然群体研究表明，该群体遗传多样性高，群体结构简单；然后我们对该群体每份种质的1棵雌株无性扩繁，建立了115份基因型一致的雌株无性系；对其CBD含量测量结果表明，CBD含量变异广泛（0.01%-4%）。表明该群体可作为CBD含量的关联分析群体。本项目拟以大麻115份雌株无性系为研究材料，利用重测序技术开发全基因组SNP标记，结合4个环境精确测定的关联分析群体CBD含量，采用全基因组关联分析法发掘与CBD含量初步候选基因，并通过qRT-PCR技术进行验证，最终获得CBD含量候选基因。本研究可深化对种质资源的认识，奠定分子辅助选择育种基础。

Cannabidiol (CBD, cannabidiol) is a kind of secondary metabolite, which is unique in hemp plants, and has important medicinal value. However,the genetic mechanism of CBD content in hemp is not clear so far. Exploring CBD content related candidate genes is the basis for molecular breeding of new varities and elucidating its genetic mechanism in hemp. Our previous study of natural germplasm population including 115 hemp accessions showed that the population had high genetic diversity and simple structure. Furtherly, we established 115 genotype-identical hemp female clones by asexually expanding of one female plant per germplasm in this population. CBD content quantification of 115 accessions showed that CBD content varied very widely in this population, which indicated this populations can be used as a correlation analysis of CBD content groups. In this study, 115 hemp accessions will be re-sequenced to develop SNP and the CBD contend will be accurate determined in four environments. We try to explore the candidate gene for CBD content from the 115 hemp accessions clones by genome-wide association analysis and verification using the qRT-PCR. This study will deepen the understanding of germplasm resources and lay the foundation for marker-assisted selection breeding.