Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties
Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties
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通过比较转录组和对比品种的生理分析揭示关键大麻盐响应基因和途径
DOI：
10.3390/agronomy11112338
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发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
Liang Zhao
中科院分区：
文献类型：
--
作者：
Jiangjiang Zhang;Zhang Cuiping;Siqi Huang;Chang Li;Jianjun Li;Huijuan Tang;Susmita Dey;Ashok Biswas;Dengxiang Du;Defang Li;Liang Zhao
为了解剖和鉴定大麻对盐胁迫的分子响应机制，对其生理特性的多样性进行了实验研究。利用RNA-seq图谱分析了不同大麻材料对盐胁迫反应的差异表达基因和途径。生理多样性分析结果表明，K94比W20对脯氨酸含量更敏感，K94需6h才能达到最大值，而W20仅需12h。在处理后0-72h，共鉴定出10149个差异表达基因，其中249个基因在植物激素信号转导和MAPK信号通路中表现出显著差异表达。共有371个基因在W20中表现出显著的差异表达变化，这些基因聚集在苯丙素生物合成和植物激素信号转导途径中。在K94和W20中发现的基因的途径丰富趋势与植物激素信号转导途径和MAPK信号通路中的基因聚集的趋势相似。此外，有85个基因识别出这两个材料之间的重叠，表明这些基因可能是与大麻中的盐胁迫有关的潜在基因。RNA-seq与qRT-PCR的符合率为86.67%，表明RNA-seq技术的准确性和可靠性。此外，生理多样性的结果与基于RNA序列的预测结果是一致的。这项研究可能为调节大麻对盐胁迫做出反应的分子网络提供新的见解。
For the dissection and identification of the molecular response mechanisms to salt stress in cannabis, an experiment was conducted surveying the diversity of physiological characteristics. RNA-seq profiling was carried out to identify differential expression genes and pathway which respond to salt stress in different cannabis materials. The result of physiological diversity analyses showed that it is more sensitive to proline contents in K94 than in W20; 6 h was needed to reach the maximum in K94, compared to 12 h in W20. For profiling 0–72 h after treatment, a total of 10,149 differentially expressed genes were identified, and 249 genes exhibited significantly diverse expression levels in K94, which were clustered in plant hormone signal transduction and the MAPK signaling pathway. A total of 371 genes showed significant diversity expression variations in W20, which were clustered in the phenylpropanoid biosynthesis and plant hormone signal transduction pathway. The pathway enrichment by genes which were identified in K94 and W20 showed a similar trend to those clustered in plant hormone signal transduction pathways and MAPK signaling. Otherwise, there were 85 genes which identified overlaps between the two materials, indicating that these may be underlying genes related to salt stress in cannabis. The 86.67% agreement of the RNA-seq and qRT-PCR indicated the accuracy and reliability of the RNA-seq technique. Additionally, the result of physiological diversity was consistent with the predicted RNA-seq-based findings. This research may offer new insights into the molecular networks mediating cannabis to respond to salt stress.
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影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
影响因子：
4.3
作者：
Adal AM;Doshi K;Holbrook L;Mahmoud SS
通讯作者：
Mahmoud SS
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
14.9
作者：
Wang L;Park HJ;Dasari S;Wang S;Kocher JP;Li W
通讯作者：
Li W
影响因子：
2.5
作者：
C. Slabu;C. Zörb;D. Steffens;S. Schubert
通讯作者：
C. Slabu;C. Zörb;D. Steffens;S. Schubert