G-四链体的形成阻止了端粒酶对端粒DNA引物的识别，抑制了端粒酶的活性表达，从而抑制肿瘤生长。因此以提高G-四链体DNA结构稳定性为目标的端粒酶抑制剂是目前抗癌药物研究的重要方向。Isatin是一种存在于经典抗癌中药青黛以及人体的内源性活性物质，对肿瘤细胞的生长具有抑制作用。本项目以G-四链体为作用靶点设计、合成具有抗肿瘤作用的Isatin新型手性衍生物及其金属配合物。通过多种谱学手段并结合分子模拟，研究金属离子以及手性因素对Isatin衍生物与G-四链体DNA的相互作用影响，得到相互作用的结合常数、结合位点、热力学参数和键合方式。研究它们对不同类型肿瘤细胞的抗癌活性，探讨结构与抗癌活性的关系，拟发现具有良好抗癌活性的先导化合物，为Isatin衍生物的新药开发奠定基础。

The formation of G-quadruplex prevents the identification of telomerase to telomeric DNA primers, inhibits the expression of telomerase activity, thereby inhibiting tumor growth. Therefore, improving the structural stability of G-quadruplex DNA of telomerase inhibitor is an important research field in anticancer drugs study. Isatin, which exists in the traditional Chinese medicine natural indigo naturalis and human body as an endogenous active substance, it is of inhibitory effect on the growth of tumor cells. In this project, the novel isatin chiral derivatives and their metal complexes which are of anti-tumor activities targeting G-quadruplex are designed and synthesized. Study on the influence of metal ions and chiral factor to the interaction between G-quadruplex DNA and Isatin derivatives; obtain the binding constants, binding sites, thermodynamic parameters and bonding way of the interaction between G-quadruplex DNA and Isatin derivatives by a variety of means of spectroscopy combined with molecular simulation.The anticancer activity and structure-activity relationships of these derivatives are researched in order to find lead compounds with good antitumor activities, the results will offer the theoretical basis for developing new drug of isatin derivatives.