通过激光辅助拉制技术及后续刻蚀方法，制备得到单一纳米盘电极、单一纳米线电极和单一纳米孔电极。运用扫描电镜、透射电镜、电化学方法和有限元模拟方法对各种形状的单一纳米电极进行表征。本项目主要利用单一纳米电极尺寸小（r < 10 nm)、传质速率快、充电电流小等优点，重点研究各种单一纳米电极在单细胞/单纳米粒子分析中的应用。主要拟开展单一纳米线电极基纳米尺寸传感器在单细胞检测等生物活体分析中的应用；纳米粒子/生物分子在单一纳米盘电极和单一纳米孔电极表面的碰撞、传输研究；新型单一纳米粒子电极在催化/能源领域的基础研究等。

Based on laser-assisted pulling technique and the following etching process, several single nanoelectrodes, including single nanodisk electrodes, single nanowire electrodes and single nanopore electrodes, were successfully fabricated. The prepared single nanoelectrodes were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), electrochemical method and finite-element simulation. This project mainly focuses on the wide applications in single cell/single nanoparticles analysis by use of single nanoelectrode due to its ultra-small size (r < 10 nm), fast transport rate and small charging current, which should include real-time single cell detection, single nanoparticle/small molecule collision and translocation, and the basic study of single nanoparticles on catalysis/energy-related field.