药用植物核心种质构建是其遗传资源有效利用的重要前提。本项目以栽培草果及其邻近分布的同属野生近缘植物拟草果和野草果为研究材料，在前期研究的基础上（包括SSR引物筛选、果实数量性状分析、繁殖生物学研究等），通过全面的野外调查取样，利用cpDNA序列和SSR微卫星标记进行群体遗传学和分子谱系地理学分析，揭示栽培草果的群体遗传结构与谱系分化程度，再利用常规植化方法结合GS-MS技术对不同栽培产地的草果种子挥发油进行代谢谱测定，探讨草果种质资源的遗传多样性与药材品质的相关性，进而完成草果核心种质的构建。目前，草果栽培起源问题仍然未知，通过分析长期栽培对草果遗传组成及其分布式样的影响，以及草果与其邻近分布的拟草果、野草果的亲缘关系和它们对草果栽培起源过程中的遗传贡献，探讨草果起源的成因。研究结果将为草果、以及拟草果和野草果遗传资源发掘和有效利用提供科学依据。

Constructing core collection of pharmaceutical plant is an important precondition for the effective utility of its genetic resources. The proposed project is based on preliminary works from our group, including SSR primers screening, quantitative character analysis of the fruit, reproductive biology research on A. tsaoko, etc. A comprehensive field investigation and sampling across A. tsaoko culture area and its wild relative (A. paratsaoko and A. koenigii) populations, distributed in the areas around the cultivated A. tsaoko, will be conducted. Based on cpDNA sequence and SSR markers, the analysis of population genetics and molecular phylogeography would explore the population genetic structure and the degree of lineage differentiation about A. tsaoko. The metabolomics fingerprints of the materia medica from different culture regions, especially the active ingredient content and variation, will be determined by conventional phytochemical techniques and GS-MS to screen for origin characteristic metabolic markers to explore the correlation between the genetic diversity of germplasm resources and the quality of materia medica. The construction of A. tsaoko core germplasm will be completed based on the results. To date, the origin of cultivated Amomum tsaoko remains unclear. It is analysized the influence of long-term cultivation on its genetic composition and the distribution pattern. Then we could assess the genetic relationships between A. tsaoko and its wild relatives to explore its cultivation origin and the potential genetic contributions from the wild relatives during the process of cultivation of A. tsaoko. The information obtained in this study will be valuable for the genetic resource exploitation and effective utility of A. tsaoko and its wild relatives, A. paratsaoko and A. koenigii.