颞叶内侧癫痫表现为慢性进展性病程且对多种抗癫痫药物耐药.近期研究发现单纯疱疹病毒6型（HHV-6B病毒）感染与MTLE相关,感染诱导的炎症因子的表达参与了海马硬化和胶质增生的病理生理过程，但炎性因子激活及衍变的机制不清。前期研究发现MTLE患者脑组织存在HHV-6B病毒活化并伴核转录因子NF-kB表达变化，据此假设HHV-6病毒感染诱导的炎性网络因子激活与MTLE慢性炎性环境形成及其耐药密切相关。因此，我们拟测定100例MTLE患者术后脑组织中HHV-6B阳性感染率，结合病毒核酸分析和病毒外壳蛋白测定明确病毒特性，并检测炎性网络上关键因子的表达水平；我们还拟在体外实验中培养星形胶质细胞，观察炎性网络中起关键作用的NF-kB阻断剂吡咯烷二硫代氨基甲酸酯(PDTC)对病毒蛋白及上述炎性因子表达水平的影响，以探讨HHV-6B病毒感染诱导炎性网络在MTLE中的作用机制。寻找潜在的药物治疗新靶点。

Temporal lobe epilepsy is a progressive neurological disorder that resistant to multiple anti-epileptic drugs. In recent studies, human herpes virus (HHV) 6B was detected in a number of patients and was verified as a promoting factor of inflammatory response associating with hippocampus sclerosis and gliosis of meisial temporal lobe epilepsy (MTLE).However, the mechanism of activating inflammatory factors is not clear till now. In our recent study where active NFkB, was associated with HHV-6 infected cells in MTLE patients. We hypothesize that the neuroinflammatory response to HHV-6B infection contributes to the chronic progression of MTLE due to activation of inflammatory factors. A research to investigate HHV-6B infection and the expression of neuroinflammatory molecules in MTLE patients will be performed in 100 samples from patients with MTLE.The activation of virus will be detected by nestPCR,IHC; the expression of inflammatory factors also will be dected. The vitro experiment of culturing astrocytes and investigatiing impact of on virus protein and inflammatory factors also will be performed. This study will determine the relationship between HHV-6B in the CNS, the chronic inflammatory environment within brain tissue and MTLE as well as identify targets for theraputics for the treatment of MTLE.