喉癌是严重威胁人类健康的疾病，尤其是晚期喉癌死亡率仍较高。喉癌发生发展的确切分子机制尚未阐明，多认为与癌基因和抑癌基因的失调有关。非编码RNA指的是不被翻译成蛋白质RNA。本课题组的前期研究筛选出喉癌中差异表达的非编码LncRNA和miRNA，但它们在喉癌中作用，以及对喉癌中表观遗传和信号传导的调控尚不清楚。课题组的预实验发现LncRNA-HOTAIR能调节PTEN基因的启动子甲基化，miR-21能调节Ras的表达。由此我们推测LncRNA和miRNA将可能通过调节表观遗传、信号传导或与蛋白结合等机制调控喉癌的发生发展。为证实这一假说，我们将运用microarray，定量PCR，凋亡实验，蛋白印迹，Chip，RNA免疫共沉淀，pull-down和生物信息分析等方法，观察LncRNA和miRNA在喉癌中的作用，并从新的视角阐明喉癌发生发展的机制。本研究将可望为喉癌治疗提供新的有效靶点和策略。

Laryngeal cancer is a malignant tumor which is seriously threatening to human health. Its mortality is still high,especially for later stage of laryngeal cancer. The precise molecular mechanisms of laryngeal cancer have not been elucidated.It is mainly considered to related with oncogene and tumor suppressor gene disorders. Non coding RNAs are RNAs that not translated to protein. Our early study shows non-coding-LncRNA and miRNA are abberantly expression in laryngeal cancer. But their regulation in epigenetic and signal transduction is unclear. Our study further suggested LncRNA-HOTAIR can modulate PTEN gene promoter methylation and miR-21 can regulate the expression of Ras. Thus we hypothesized that LncRNA and miRNA should regulate the occurrence and development of laryngeal carcinoma through epigenetic and signal transduction mechanism. To confirm this hypothesis, we will use the methods of microarray, qRT-PCR, apoptosis assay, Western-blot, Chip and RNA-chip， RNA-pull-down and bioinformatics analysis methods to observe the role of LncRNA and miRNA in laryngeal cancer and clarify the mechanism of laryngeal cancer development from a new perspective. This study is expected to provide a new effective target and strategy for therapy of laryngeal cancer.