喉癌是严重威胁人类健康的疾病，尤其是晚期喉癌死亡率仍较高，因此，寻找喉癌可靠的分子marker及阐明其分子机制迫在眉睫。非编码RNA在肿瘤进程中起到重要的调控作用。本课题组的前期研究在miRNA、lncRNA和circRNA中筛选出喉癌组织和血液中显著高表达的lncRNA-RP11-159K7.2，并首次证明其参与了喉癌的DNA甲基化调控。但它能否作为喉癌可靠的分子marker，以及它调控DNA甲基化的机制尚不清楚。为阐明这一科学问题，我们将检测大样本喉癌组织、血清及外泌体中RP11-159K7.2的表达，观察其与复发、转移和预后的关系。进一步运用基因转染，CRISPR/Cas9，侵袭实验，荧光素酶，RIP，pulldown等方法，从新的视角揭示RP11-159K7.2在喉癌中的作用及其调控DNA甲基化的机制。本项目可望为喉癌预警、预后评估及治疗提供新的靶点和策略。

Laryngeal carcinoma is a malignant tumour with high mortality in the advanced stage, which seriously threaten human health. It is urgent to search for reliable molecular markers of laryngeal carcinoma and elucidate the molecular mechanisms of the disease. Non-coding RNA plays a significant regulatory role in carcinogenesis process. Our research group has screened out lncRNA-RP11-159K7.2 from substantial miRNAs, lncRNAs and circRNAs which are significantly higher expressed in tumour tissue and blood of laryngeal carcinoma. Besides, we proved that it is involved in the regulation of DNA methylation in laryngeal carcinoma for the first time. However, it is not clear whether RP11-159K7.2 can be used as a reliable molecular biomarker, and the regulation mechanism of DNA methylation which RP11-159K7.2 involved in still needs further study. In order to clarify this problem, we intend to detect the expression of RP11-159K7.2 in large sample of laryngeal carcinoma tissues, serum and exosome, and observe its relationship with the metastasis, recurrence and prognosis of the malignancy. Using gene transfection, CRISPR/Cas9, invasion experiment, luciferase experiment, RIP, pulldown and other methods, we will reveal the role of RP11-159K7.2 in laryngeal carcinoma, and its regulation mechanisms of DNA methylation from a new perspective. This project is expected to provide a new target and strategy for early warning, prognosis evaluation and treatment of laryngeal carcinoma.