雷帕霉素靶蛋白（mTOR）是一种丝/苏氨酸蛋白激酶，介导细胞内信号转导，而ATP敏感钾（KATP）通道是内向整流钾离子通道，两者均具有诸多重要的神经调节功能。近年发现激活KATP通道能促进细胞内多种信号通路的交互作用。已有证据显示mTOR信号通过提高KATP通道活动而抑制下丘脑POMC神经元的活动。皮层运动区深层L5锥体神经元在运动学习中起着关键作用，成熟后多数成为爆发式放电（BS）类型，这种特征与运动发育学习的关系不明确。我们拟选择性地干扰L5神经元mTOR-KATP途径，综合应用分子生物学、电生理学、形态学和行为学等多种研究手段，以揭示mTOR信号与KATP通道的相互作用及机制，明确其年龄依赖性地调控BS神经元爆发式放电的特性，及其对运动发育学习能力的调控作用。研究结果将有助于人们更好了解mTOR-KATP途径对脑功能活动的调节作用、了解运动发育学习能力的神经生物学机制和意义。

Mammalian target of rapamycin (mTOR) is a serine / threonine protein kinase, mediating intracellular signal transduction, while ATP-sensitive potassium (KATP) channel is a membrane channel protein sensing the metabolic state of a cell to its electrical activity, both of which have many important neural regulatory effects. In recent years, increasing evidence shows that the activation of KATP channels could promote the cross-talks among multiple intracellular signaling pathways. It has been demonstrated that the mTOR signaling increase the activity of KATP channels to inhibit the electrical activity of the hypothalamic POMC neuron. Motor cortex deep layer such as L5 pyramidal neurons play a key role in the motor development and motor learning. As most of the L5 pyramidal neurons develop as the burst spiking (BS) neurons in the mature brain, its relationship to the motor development and motor learn ability is not clear. We plan to selectively interfere with mTOR-KATP pathway of the L5 neurons, applying tools of molecular biology, electrophysiology, morphology and behavioral assessments to reveal ① mTOR-KATP pathway expressions correlate to the electrophysiological properties of the BS neurons in an age-dependent manner; ②mTOR-KATP pathway mediates the electrical discharge characteristics of the BS neurons; ③The regulatary molecular mechanisms underline the effects of mTOR-KATP pathway on a BS neuron; ④mTOR-KATP pathway's regulation on electrophysiological properties of the BS neurons controls the motor development and motor learning ability. Our findings will deepen the understanding of the mTOR-KATP pathway in regulating the neuronal activity and the brain function, particularly for the understanding of the neurobiological mechanisms of motor development and motor learning ability.